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XB-ART-41954
Dev Biol November 1, 2010; 347 (1): 180-94.

Sumoylation controls retinal progenitor proliferation by repressing cell cycle exit in Xenopus laevis.

Terada K , Furukawa T .


Abstract
Precisely controlled progenitor proliferation is essential for normal development. However, molecular mechanisms, which control the correct timing of cell cycle withdrawal during development, have been poorly understood. We show here that ubc9, a sumo-conjugating enzyme, controls the cell cycle exit of retinal progenitors. We found that ubc9 is highly expressed in retinal progenitors and stem cells in Xenopus embryos. Ubc9 physically and functionally associates with Xenopus hmgb3, which is required for retinal cell proliferation, and prolonged expression of ubc9 and hmgb3 results in suppression of the cell cycle exit of retinal progenitors in a sumoylation-dependent manner. Overexpression of ubc9 and hmgb3 decreased expression of the cell-cycle inhibitor p27(Xic1). Furthermore, progenitor proliferation is regulated, at least in part, by sumoylation of transcription factor Sp1. These results suggest a significant role of sumoylation for cell cycle regulation in retinal progenitors.

PubMed ID: 20801111
Article link: Dev Biol

Genes referenced: calb1 cdknx gadd45g gal.2 hmgb3 isl1 pax6 pcna rax six3 six6 sp1 ube2i vsx1 znrd2
Antibodies: Brdu Ab4 Calb1 Ab4 FLAG Ab1 H3f3a Ab9 HA Ab6 Isl1/2 Ab1 Myc Ab5 Pcna Ab1
Morpholinos: hmgb3 MO1


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