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XB-ART-42016
Proc Natl Acad Sci U S A September 14, 2010; 107 (37): 16148-53.

Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma.

Koebernick K , Loeber J , Arthur PK , Tarbashevich K , Pieler T .


Abstract
Segregation of the future germ line defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance.

PubMed ID: 20805475
PMC ID: PMC2941339
Article link: Proc Natl Acad Sci U S A

Genes referenced: cat2 dazl ddx25 dnd1 elavl1 fam102a gdf1 mbd2 nanos1 nif nos1 pgat pgc rrad ska2 vegt
Morpholinos: dnd1 MO2 dnd1 MO3 dnd1 MO4 dnd1 MO5 fam102a MO1 mbd2 MO1 rrad MO1 ska2 MO2


Article Images: [+] show captions
References [+] :
Arthur, Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis. 2009, Pubmed, Xenbase


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