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RNA
2010 Nov 01;1611:2094-107. doi: 10.1261/rna.2295410.
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Distinct functions of maternal and somatic Pat1 protein paralogs.
Marnef A
,
Maldonado M
,
Bugaut A
,
Balasubramanian S
,
Kress M
,
Weil D
,
Standart N
.
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We previously identified Xenopus Pat1a (P100) as a member of the maternal CPEB RNP complex, whose components resemble those of P-(rocessing) bodies, and which is implicated in translational control in Xenopus oocytes. Database searches have identified Pat1a proteins in other vertebrates, as well as paralogous Pat1b proteins. Here we characterize Pat1 proteins, which have no readily discernable sequence features, in Xenopus oocytes, eggs, and early embryos and in human tissue culture cells. xPat1a and 1b have essentially mutually exclusive expression patterns in oogenesis and embryogenesis. xPat1a is degraded during meiotic maturation, via PEST-like regions, while xPat1b mRNA is translationally activated at GVBD by cytoplasmic polyadenylation. Pat1 proteins bind RNA in vitro, via a central domain, with a preference for G-rich sequences, including the NRAS 5' UTR G-quadruplex-forming sequence. When tethered to reporter mRNA, both Pat proteins repress translation in oocytes. Indeed, both epitope-tagged proteins interact with the same components of the CPEB RNP complex, including CPEB, Xp54, eIF4E1b, Rap55B, and ePAB. However, examining endogenous protein interactions, we find that in oocytes only xPat1a is a bona fide component of the CPEB RNP, and that xPat1b resides in a separate large complex. In tissue culture cells, hPat1b localizes to P-bodies, while mPat1a-GFP is either found weakly in P-bodies or disperses P-bodies in a dominant-negative fashion. Altogether we conclude that Pat1a and Pat1b proteins have distinct functions, mediated in separate complexes. Pat1a is a translational repressor in oocytes in a CPEB-containing complex, and Pat1b is a component of P-bodies in somatic cells.
Balagopal,
Stm1 modulates mRNA decay and Dhh1 function in Saccharomyces cerevisiae.
2009, Pubmed
Balagopal,
Stm1 modulates mRNA decay and Dhh1 function in Saccharomyces cerevisiae.
2009,
Pubmed
Beckham,
P bodies, stress granules, and viral life cycles.
2008,
Pubmed
Beilharz,
microRNA-mediated messenger RNA deadenylation contributes to translational repression in mammalian cells.
2009,
Pubmed
Bhattacharyya,
Relief of microRNA-mediated translational repression in human cells subjected to stress.
2006,
Pubmed
Boag,
Protection of specific maternal messenger RNAs by the P body protein CGH-1 (Dhh1/RCK) during Caenorhabditis elegans oogenesis.
2008,
Pubmed
Bouveret,
A Sm-like protein complex that participates in mRNA degradation.
2000,
Pubmed
Braun,
The C-terminal alpha-alpha superhelix of Pat is required for mRNA decapping in metazoa.
2010,
Pubmed
Chable-Bessia,
Suppression of HIV-1 replication by microRNA effectors.
2009,
Pubmed
Chu,
Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54.
2006,
Pubmed
Coller,
General translational repression by activators of mRNA decapping.
2005,
Pubmed
Copeland,
The mechanism and regulation of deadenylation: identification and characterization of Xenopus PARN.
2001,
Pubmed
,
Xenbase
Cougot,
Cytoplasmic foci are sites of mRNA decay in human cells.
2004,
Pubmed
Darnell,
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
2001,
Pubmed
Decker,
Edc3p and a glutamine/asparagine-rich domain of Lsm4p function in processing body assembly in Saccharomyces cerevisiae.
2007,
Pubmed
Ding,
Repression of C. elegans microRNA targets at the initiation level of translation requires GW182 proteins.
2009,
Pubmed
Ernoult-Lange,
Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies.
2009,
Pubmed
Eulalio,
P bodies: at the crossroads of post-transcriptional pathways.
2007,
Pubmed
Eulalio,
P-body formation is a consequence, not the cause, of RNA-mediated gene silencing.
2007,
Pubmed
Eulalio,
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing.
2007,
Pubmed
Fabian,
Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.
2009,
Pubmed
Flemr,
P-body loss is concomitant with formation of a messenger RNA storage domain in mouse oocytes.
2010,
Pubmed
Gallo,
Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos.
2008,
Pubmed
Gillian-Daniel,
Modifications of the 5' cap of mRNAs during Xenopus oocyte maturation: independence from changes in poly(A) length and impact on translation.
1998,
Pubmed
,
Xenbase
Gray,
Multiple portions of poly(A)-binding protein stimulate translation in vivo.
2000,
Pubmed
,
Xenbase
Haas,
HPat provides a link between deadenylation and decapping in metazoa.
2010,
Pubmed
Hake,
Specificity of RNA binding by CPEB: requirement for RNA recognition motifs and a novel zinc finger.
1998,
Pubmed
,
Xenbase
Jackson,
The mechanism of eukaryotic translation initiation and principles of its regulation.
2010,
Pubmed
Jackson,
Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA.
1983,
Pubmed
Kedersha,
Mammalian stress granules and processing bodies.
2007,
Pubmed
Kotaja,
The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components.
2006,
Pubmed
Kumari,
An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene modulates translation.
2007,
Pubmed
Marnef,
Pat1 proteins: a life in translation, translation repression and mRNA decay.
2010,
Pubmed
Minshall,
Translational control assessed using the tethered function assay in Xenopus oocytes.
2010,
Pubmed
,
Xenbase
Minshall,
A conserved role of a DEAD box helicase in mRNA masking.
2001,
Pubmed
,
Xenbase
Minshall,
The active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer.
2004,
Pubmed
,
Xenbase
Minshall,
CPEB interacts with an ovary-specific eIF4E and 4E-T in early Xenopus oocytes.
2007,
Pubmed
,
Xenbase
Minshall,
Role of p54 RNA helicase activity and its C-terminal domain in translational repression, P-body localization and assembly.
2009,
Pubmed
,
Xenbase
Nakamura,
Translational repression by the oocyte-specific protein P100 in Xenopus.
2010,
Pubmed
,
Xenbase
Nathans,
Cellular microRNA and P bodies modulate host-HIV-1 interactions.
2009,
Pubmed
Noble,
Maternal mRNAs are regulated by diverse P body-related mRNP granules during early Caenorhabditis elegans development.
2008,
Pubmed
Ozgur,
Human Pat1b connects deadenylation with mRNA decapping and controls the assembly of processing bodies.
2010,
Pubmed
Parker,
P bodies and the control of mRNA translation and degradation.
2007,
Pubmed
Pilkington,
Pat1 contains distinct functional domains that promote P-body assembly and activation of decapping.
2008,
Pubmed
Radford,
Translational control by cytoplasmic polyadenylation in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Rechsteiner,
PEST sequences and regulation by proteolysis.
1996,
Pubmed
Reverte,
CPEB degradation during Xenopus oocyte maturation requires a PEST domain and the 26S proteasome.
2001,
Pubmed
,
Xenbase
Richter,
CPEB: a life in translation.
2007,
Pubmed
,
Xenbase
Rother,
Purification, primary structure, bacterial expression and subcellular distribution of an oocyte-specific protein in Xenopus.
1992,
Pubmed
,
Xenbase
Röper,
Rtnl1 is enriched in a specialized germline ER that associates with ribonucleoprotein granule components.
2007,
Pubmed
Schaeffer,
The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif.
2001,
Pubmed
,
Xenbase
Scheller,
Identification of PatL1, a human homolog to yeast P body component Pat1.
2007,
Pubmed
Serman,
GW body disassembly triggered by siRNAs independently of their silencing activity.
2007,
Pubmed
Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase
Sonenberg,
Regulation of translation initiation in eukaryotes: mechanisms and biological targets.
2009,
Pubmed
Stalder,
Processing bodies are not required for mammalian nonsense-mediated mRNA decay.
2009,
Pubmed
Standart,
Translational control in early development: CPEB, P-bodies and germinal granules.
2008,
Pubmed
,
Xenbase
Sweet,
Microtubule disruption stimulates P-body formation.
2007,
Pubmed
Swetloff,
Dcp1-bodies in mouse oocytes.
2009,
Pubmed
Tanaka,
RAP55, a cytoplasmic mRNP component, represses translation in Xenopus oocytes.
2006,
Pubmed
,
Xenbase
Tharun,
Yeast Sm-like proteins function in mRNA decapping and decay.
2000,
Pubmed
Thom,
Role of cdc2 kinase phosphorylation and conserved N-terminal proteolysis motifs in cytoplasmic polyadenylation-element-binding protein (CPEB) complex dissociation and degradation.
2003,
Pubmed
,
Xenbase
Van Dyke,
Stm1p, a G4 quadruplex and purine motif triplex nucleic acid-binding protein, interacts with ribosomes and subtelomeric Y' DNA in Saccharomyces cerevisiae.
2004,
Pubmed
Voeltz,
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Wilczynska,
The translational regulator CPEB1 provides a link between dcp1 bodies and stress granules.
2005,
Pubmed
,
Xenbase
Wyers,
Deletion of the PAT1 gene affects translation initiation and suppresses a PAB1 gene deletion in yeast.
2000,
Pubmed
Yu,
Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body.
2005,
Pubmed
Zanotti,
Thermodynamics of the fragile X mental retardation protein RGG box interactions with G quartet forming RNA.
2006,
Pubmed
Zekri,
The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release.
2009,
Pubmed
Zhang,
Monitoring mRNA decapping activity.
1999,
Pubmed
,
Xenbase
