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Dev Biol January 1, 2004; 265 (1): 169-80.

Neurotrophin-independent attraction of growing sensory and motor axons towards developing Xenopus limb buds in vitro.

Tonge DA , Pountney DJ , Leclere PG , Zhu N , Pizzey JA .

The mechanisms for directing axons to their targets in developing limbs remain largely unknown though recent studies in mice have demonstrated the importance of neurotrophins in this process. We now report that in co-cultures of larval Xenopus laevis limb buds with spinal cords and dorsal root ganglia of Xenopus and axolotl (Ambystoma mexicanum) axons grow directly to the limb buds over distances of up to 800 microm and in particular to sheets of epidermal cells which migrate away from the limb buds and also tail segments in culture. This directed axonal growth persists in the presence of trk-IgG chimeras, which sequester neurotrophins, and k252a, which blocks their actions mediated via trk receptors. These findings indicate that developing limb buds in Xenopus release diffusible factors other than neurotrophins, able to attract growth of sensory and motor axons over long distances.

PubMed ID: 14697361
Article link: Dev Biol

Species referenced: Xenopus laevis
Genes referenced: acod1lb bdnf bmp2 gdnf h3-3a hgf krt62 ngf ntf3 ntf4 ntrk1 ntrk2 ntrk3 snai2
Antibodies: H3f3a Ab9 Tuba4b Ab4

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