Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-42161
Dev Dyn December 1, 2010; 239 (12): 3172-81.

Characterization of a novel type I keratin gene and generation of transgenic lines with fluorescent reporter genes driven by its promoter/enhancer in Xenopus laevis.

Suzuki KT , Kashiwagi K , Ujihara M , Marukane T , Tazaki A , Watanabe K , Mizuno N , Ueda Y , Kondoh H , Kashiwagi A , Mochii M .


Abstract
We investigated the characteristics of a novel type I keratin gene in Xenopus laevis during ontogenesis. The transcript was first detected in the posterior region at the late neurula stage, and then restricted to the fin and external gill during embryogenesis. To examine the transcriptional regulation of the keratin gene in vivo, we generated transgenic lines with fluorescent reporter genes driven by its 4.2-kb upstream sequence. The promoter/enhancer activity recapitulated the endogenous gene expression during embryogenesis. Sequential deletion analyses revealed that the regions proximal to the promoter were essential for fin-specific expression. Reporter expression was detected in various organs, including the fin and gill. In particular, robust expression was observed in the developing limbs and gill. The reporter fluorescence rapidly decreased with internal gill resorption during metamorphosis. The transgenic lines carrying the promoter/enhancer should represent valuable tools for elucidating the formation, development and resorption of various organs, especially the gill.

PubMed ID: 20941778
Article link: Dev Dyn


Species referenced: Xenopus laevis
Genes referenced: krt12.2 krt12.4 krt12.5 krt12.6 krt15.1 krt15.2 krt17 krt18.1 krt19 krt55 krt62 krt70


Article Images: [+] show captions