Due to necessary maintenance, Xenbase will be unavailable from December 24-29, 2014. We apologize for the inconvenience.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-42284
Science. October 8, 2010; 330 (6001): 231-5.

Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.

Wang F , Dai J , Daum JR , Niedzialkowska E , Banerjee B , Stukenberg PT , Gorbsky GJ , Higgins JM .


Abstract
Aurora B is a component of the chromosomal passenger complex (CPC) required for correct spindle-kinetochore attachments during chromosome segregation and for cytokinesis. The chromatin factors that recruit the CPC to centromeres are unknown, however. Here we show that phosphorylation of histone H3 threonine 3 (H3T3ph) by Haspin is necessary for CPC accumulation at centromeres and that the CPC subunit Survivin binds directly to H3T3ph. A nonbinding Survivin-D70A/D71A mutant does not support centromeric CPC concentration, and both Haspin depletion and Survivin-D70A/D71A mutation diminish centromere localization of the kinesin MCAK and the mitotic checkpoint response to taxol. Survivin-D70A/D71A mutation and microinjection of H3T3ph-specific antibody both compromise centromeric Aurora B functions but do not prevent cytokinesis. Therefore, H3T3ph generated by Haspin positions the CPC at centromeres to regulate selected targets of Aurora B during mitosis.

PubMed ID: 20705812
PMC ID: PMC2967368
Article link: Science.
Grant support: R01-GM050412 NIGMS NIH HHS , R01-GM063045 NIGMS NIH HHS , R01-GM074210 NIGMS NIH HHS , R01 GM074210-04 NIGMS NIH HHS , R01 GM050412-16 NIGMS NIH HHS , R01 GM063045-10 NIGMS NIH HHS , R01 GM050412-18 NIGMS NIH HHS

Genes referenced: aurkb birc5.1 kif2c
Antibodies referenced:
Morpholinos referenced:

My Xenbase: [ Log-in / Register ]
version: [3.3]


Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556