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Science
2010 Oct 08;3306001:231-5. doi: 10.1126/science.1189435.
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Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.
Wang F
,
Dai J
,
Daum JR
,
Niedzialkowska E
,
Banerjee B
,
Stukenberg PT
,
Gorbsky GJ
,
Higgins JM
.
???displayArticle.abstract??? Aurora B is a component of the chromosomal passenger complex (CPC) required for correct spindle-kinetochore attachments during chromosome segregation and for cytokinesis. The chromatin factors that recruit the CPC to centromeres are unknown, however. Here we show that phosphorylation of histone H3 threonine 3 (H3T3ph) by Haspin is necessary for CPC accumulation at centromeres and that the CPC subunit Survivin binds directly to H3T3ph. A nonbinding Survivin-D70A/D71A mutant does not support centromeric CPC concentration, and both Haspin depletion and Survivin-D70A/D71A mutation diminish centromere localization of the kinesin MCAK and the mitotic checkpoint response to taxol. Survivin-D70A/D71A mutation and microinjection of H3T3ph-specific antibody both compromise centromeric Aurora B functions but do not prevent cytokinesis. Therefore, H3T3ph generated by Haspin positions the CPC at centromeres to regulate selected targets of Aurora B during mitosis.
Andrews,
Aurora B regulates MCAK at the mitotic centromere.
2004, Pubmed
Andrews,
Aurora B regulates MCAK at the mitotic centromere.
2004,
Pubmed
Cao,
Survivin mutant (Surv-DD70, 71AA) disrupts the interaction of Survivin with Aurora B and causes multinucleation in HeLa cells.
2006,
Pubmed
Carvalho,
Survivin is required for stable checkpoint activation in taxol-treated HeLa cells.
2003,
Pubmed
Dai,
Regulation of mitotic chromosome cohesion by Haspin and Aurora B.
2006,
Pubmed
Dai,
The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.
2005,
Pubmed
Ditchfield,
Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores.
2003,
Pubmed
Gassmann,
Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.
2004,
Pubmed
Hsu,
Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
2000,
Pubmed
Huang,
Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments.
2007,
Pubmed
Jeyaprakash,
Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together.
2007,
Pubmed
Kawashima,
Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres.
2007,
Pubmed
Klein,
Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
2006,
Pubmed
Lan,
Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity.
2004,
Pubmed
,
Xenbase
Lavogina,
Co-crystal structures of the protein kinase haspin with bisubstrate inhibitors.
2016,
Pubmed
Lens,
Uncoupling the central spindle-associated function of the chromosomal passenger complex from its role at centromeres.
2006,
Pubmed
Lens,
Survivin is required for a sustained spindle checkpoint arrest in response to lack of tension.
2003,
Pubmed
Liu,
Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates.
2009,
Pubmed
McGuinness,
Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells.
2005,
Pubmed
Musacchio,
Molecular biology. Surfing chromosomes (and Survivin).
2010,
Pubmed
Ohi,
Differentiation of cytoplasmic and meiotic spindle assembly MCAK functions by Aurora B-dependent phosphorylation.
2004,
Pubmed
,
Xenbase
Rosasco-Nitcher,
Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation.
2008,
Pubmed
,
Xenbase
Ruchaud,
Chromosomal passengers: conducting cell division.
2007,
Pubmed
Salic,
Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis.
2004,
Pubmed
,
Xenbase
Song,
Direct interaction between survivin and Smac/DIABLO is essential for the anti-apoptotic activity of survivin during taxol-induced apoptosis.
2003,
Pubmed
Srinivasula,
IAPs: what's in a name?
2008,
Pubmed
Sun,
Solution structure of human survivin and its binding interface with Smac/Diablo.
2005,
Pubmed
Vader,
Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody.
2006,
Pubmed
Vermaak,
Multiple roles for heterochromatin protein 1 genes in Drosophila.
2009,
Pubmed
Vong,
Chromosome alignment and segregation regulated by ubiquitination of survivin.
2005,
Pubmed
,
Xenbase
Welburn,
Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface.
2010,
Pubmed
Yue,
Deconstructing Survivin: comprehensive genetic analysis of Survivin function by conditional knockout in a vertebrate cell line.
2008,
Pubmed
Zeitlin,
CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis.
2001,
Pubmed
