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Mol Cell
2010 Dec 10;405:834-40. doi: 10.1016/j.molcel.2010.11.027.
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Uncoupling of sister replisomes during eukaryotic DNA replication.
Yardimci H
,
Loveland AB
,
Habuchi S
,
van Oijen AM
,
Walter JC
.
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The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that they travel away from one another and thus function independently. Alternatively, sister replisomes may form a stationary, functional unit that draws parental DNA toward itself. If this "double replisome" model is correct, a constrained DNA molecule should not undergo replication. To test this prediction, lambda DNA was stretched and immobilized at both ends within a microfluidic flow cell. Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication by a single pair of diverging replisomes. The data show that there is no obligatory coupling between sister replisomes and, together with other studies, imply that genome duplication involves autonomously functioning replisomes.
Alexandrov,
Characterization of simian virus 40 T-antigen double hexamers bound to a replication fork. The active form of the helicase.
2002, Pubmed
Alexandrov,
Characterization of simian virus 40 T-antigen double hexamers bound to a replication fork. The active form of the helicase.
2002,
Pubmed
Blow,
Replication origins in Xenopus egg extract Are 5-15 kilobases apart and are activated in clusters that fire at different times.
2001,
Pubmed
,
Xenbase
Bochman,
The Mcm complex: unwinding the mechanism of a replicative helicase.
2009,
Pubmed
Botchan,
DNA replication: making two forks from one prereplication complex.
2010,
Pubmed
COHEN,
SPECTROPHOTOMETRIC STUDIES OF THE INTERACTION OF CHLOROQUINE WITH DEOXYRIBONUCLEIC ACID.
1965,
Pubmed
Conti,
Replication fork velocities at adjacent replication origins are coordinately modified during DNA replication in human cells.
2007,
Pubmed
Doksani,
Replicon dynamics, dormant origin firing, and terminal fork integrity after double-strand break formation.
2009,
Pubmed
Dubey,
Do sister forks of bidirectionally growing replicons proceed at unequal rates?
1987,
Pubmed
Edwards,
MCM2-7 complexes bind chromatin in a distributed pattern surrounding the origin recognition complex in Xenopus egg extracts.
2002,
Pubmed
,
Xenbase
Evrin,
A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication.
2009,
Pubmed
,
Xenbase
Falaschi,
Eukaryotic DNA replication: a model for a fixed double replisome.
2000,
Pubmed
Fletcher,
The structure and function of MCM from archaeal M. Thermoautotrophicum.
2003,
Pubmed
Gambus,
GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks.
2006,
Pubmed
Herrick,
Replication fork density increases during DNA synthesis in X. laevis egg extracts.
2000,
Pubmed
,
Xenbase
Ilves,
Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.
2010,
Pubmed
Kitamura,
Live-cell imaging reveals replication of individual replicons in eukaryotic replication factories.
2006,
Pubmed
Lebofsky,
DNA is a co-factor for its own replication in Xenopus egg extracts.
2011,
Pubmed
,
Xenbase
Lee,
DNA primase acts as a molecular brake in DNA replication.
2006,
Pubmed
Ligasová,
Organization of human replicon: singles or zipping couples?
2009,
Pubmed
Lu,
Histone H1 reduces the frequency of initiation in Xenopus egg extract by limiting the assembly of prereplication complexes on sperm chromatin.
1998,
Pubmed
,
Xenbase
Mahbubani,
DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Moyer,
Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase.
2006,
Pubmed
Remus,
Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
2009,
Pubmed
Reyes-Lamothe,
Independent positioning and action of Escherichia coli replisomes in live cells.
2008,
Pubmed
Sclafani,
Two heads are better than one: regulation of DNA replication by hexameric helicases.
2004,
Pubmed
Smith,
The bacteriophage straight phi29 portal motor can package DNA against a large internal force.
2001,
Pubmed
Takahashi,
Pumps, paradoxes and ploughshares: mechanism of the MCM2-7 DNA helicase.
2005,
Pubmed
Takahashi,
Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex.
2004,
Pubmed
,
Xenbase
Tapper,
Preferred DNA sites are involved in the arrest and initiation of DNA synthesis during replication of SV40 DNA.
1980,
Pubmed
Valle,
Large T-antigen double hexamers imaged at the simian virus 40 origin of replication.
2000,
Pubmed
Walter,
Regulated chromosomal DNA replication in the absence of a nucleus.
1998,
Pubmed
,
Xenbase
Walter,
Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha.
2000,
Pubmed
,
Xenbase
Weisshart,
Two regions of simian virus 40 T antigen determine cooperativity of double-hexamer assembly on the viral origin of DNA replication and promote hexamer interactions during bidirectional origin DNA unwinding.
1999,
Pubmed
Wessel,
Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication.
1992,
Pubmed
van Mameren,
Unraveling the structure of DNA during overstretching by using multicolor, single-molecule fluorescence imaging.
2009,
Pubmed
