Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
J Cell Biol. December 27, 2010; 191 (7): 1239-49.

A computational model predicts Xenopus meiotic spindle organization.

Loughlin R , Heald R , Nédélec F .

The metaphase spindle is a dynamic bipolar structure crucial for proper chromosome segregation, but how microtubules (MTs) are organized within the bipolar architecture remains controversial. To explore MT organization along the pole-to-pole axis, we simulated meiotic spindle assembly in two dimensions using dynamic MTs, a MT cross-linking force, and a kinesin-5-like motor. The bipolar structures that form consist of antiparallel fluxing MTs, but spindle pole formation requires the addition of a NuMA-like minus-end cross-linker and directed transport of MT depolymerization activity toward minus ends. Dynamic instability and minus-end depolymerization generate realistic MT lifetimes and a truncated exponential MT length distribution. Keeping the number of MTs in the simulation constant, we explored the influence of two different MT nucleation pathways on spindle organization. When nucleation occurs throughout the spindle, the simulation quantitatively reproduces features of meiotic spindles assembled in Xenopus egg extracts.

PubMed ID: 21173114
PMC ID: PMC3010074
Article link: J Cell Biol.
Grant support: DP1 OD000818 NCCDPHP CDC HHS, DP1 OD000818 NIH HHS , R01 GM098766 NIGMS NIH HHS , R01 DK027847 NIDDK NIH HHS , R01-DK27847 NIDDK NIH HHS

Genes referenced: numa1

External Resources:
Article Images: [+] show captions

Athale, 2008, Pubmed, Xenbase[+]

Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.9.0
Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556