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XB-ART-42958
Dev Dyn December 1, 2010; 239 (12): 3467-80.

Microarray identification of novel downstream targets of FoxD4L1/D5, a critical component of the neural ectodermal transcriptional network.



Abstract
FoxD4L1/D5 is a forkhead transcription factor that functions as both a transcriptional activator and repressor. FoxD4L1/D5 acts upstream of several other neural transcription factors to maintain neural fate, regulate neural plate patterning, and delay the expression of neural differentiation factors. To identify a more complete list of downstream genes that participate in these earliest steps of neural ectodermal development, we carried out a microarray analysis comparing gene expression in control animal cap ectodermal explants (ACs), which will form epidermis, to that in FoxD4L1/D5-expressing ACs. Forty-four genes were tested for validation by RT-PCR of ACs and/or in situ hybridization assays in embryos; 86% of those genes up-regulated and 100% of those genes down-regulated in the microarray were altered accordingly in one of these independent assays. Eleven of these 44 genes are of unknown function, and we provide herein their developmental expression patterns to begin to reveal their roles in ectodermal development.

PubMed ID: 21069826
PMC ID: PMC3057538
Article link: Dev Dyn
Grant support: [+]
Genes referenced: acss2.2 ag1 ascl1 bmp4 ccl20 cldn5 dlx3 dlx6 egr1 eif5b elf1 fgf8 fgfr2 fgfr4 foxb1 foxd4l1.1 foxi1 foxl1 gal.2 gata2 gata3 gdf3 hmox1 klf17 klf4 krt70 lef1 lhx5 mab21l3 mafb mtor nradd pou2f1 prdm1 prickle1 slc43a2 sp5l szl tfap2a tmem69 uckl1 vegt vill xmc


Article Images: [+] show captions
References:
Bertwistle, 1997, Pubmed, Xenbase [+]


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