XB-ART-43015Methods Mol Biol. January 1, 2011; 714 71-82.
Visualization of mRNA localization in Xenopus oocytes.
Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution.
PubMed ID: 21431735
PMC ID: PMC3181151
Article link: Methods Mol Biol.
Grant support: R01GM071049 NIGMS NIH HHS , R01 GM071049-17 NIGMS NIH HHS , T32 GM007601-33 NIGMS NIH HHS , T32 GM007601 NIGMS NIH HHS , R01 GM071049 NIGMS NIH HHS , 5P30ES005605 NIEHS NIH HHS , GM067841 NIGMS NIH HHS , T32 GM007601 NIGMS NIH HHS , R01 GM071049 NIGMS NIH HHS , T32 GM007601-33 NIGMS NIH HHS , R01 GM071049-17 NIGMS NIH HHS , R01GM071049 NIGMS NIH HHS