XB-ART-43041
Dev Biol
2011 Jun 15;3542:208-20. doi: 10.1016/j.ydbio.2011.03.026.
Show Gene links
Show Anatomy links
Essential roles of fibronectin in the development of the left-right embryonic body plan.
Pulina MV
,
Hou SY
,
Mittal A
,
Julich D
,
Whittaker CA
,
Holley SA
,
Hynes RO
,
Astrof S
.
???displayArticle.abstract???
Studies in Xenopus laevis suggested that cell-extracellular matrix (ECM) interactions regulate the development of the left-right axis of asymmetry; however, the identities of ECM components and their receptors important for this process have remained unknown. We discovered that FN is required for the establishment of the asymmetric gene expression pattern in early mouse embryos by regulating morphogenesis of the node, while cellular fates of the nodal cells, canonical Wnt and Shh signaling within the node were not perturbed by the absence of FN. FN is also required for the expression of Lefty 1/2 and activation of SMADs 2 and 3 at the floor plate, while cell fate specification of the notochord and the floor plate, as well as signaling within and between these two embryonic organizing centers remained intact in FN-null mutants. Furthermore, our experiments indicate that a major cell surface receptor for FN, integrin α5β1, is also required for the development of the left-right asymmetry, and that this requirement is evolutionarily conserved in fish and mice. Taken together, our studies demonstrate the requisite role for a structural ECM protein and its integrin receptor in the development of the left-right axis of asymmetry in vertebrates.
???displayArticle.pubmedLink??? 21466802
???displayArticle.pmcLink??? PMC3225965
???displayArticle.link??? Dev Biol
???displayArticle.grants??? [+]
Species referenced: Xenopus laevis
Genes referenced: fn1 lefty nodal nodal1 shh
References [+] :
Ablooglu,
Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry.
2010, Pubmed
Ablooglu, Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry. 2010, Pubmed
Amack, Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish. 2007, Pubmed , Xenbase
Astrof, Heart development in fibronectin-null mice is governed by a genetic modifier on chromosome four. 2007, Pubmed
Astrof, Multiple cardiovascular defects caused by the absence of alternatively spliced segments of fibronectin. 2007, Pubmed
Bader, Extensive vasculogenesis, angiogenesis, and organogenesis precede lethality in mice lacking all alpha v integrins. 1998, Pubmed
Bamford, Loss-of-function mutations in the EGF-CFC gene CFC1 are associated with human left-right laterality defects. 2000, Pubmed
Brennan, Nodal activity in the node governs left-right asymmetry. 2002, Pubmed
Caspary, The graded response to Sonic Hedgehog depends on cilia architecture. 2007, Pubmed , Xenbase
Chan, α8-integrins are required for hippocampal long-term potentiation but not for hippocampal-dependent learning. 2010, Pubmed
DasGupta, Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation. 1999, Pubmed
Davidson, Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension. 2006, Pubmed , Xenbase
Davidson, Live imaging of cell protrusive activity, and extracellular matrix assembly and remodeling during morphogenesis in the frog, Xenopus laevis. 2008, Pubmed , Xenbase
Davidson, Mesendoderm extension and mantle closure in Xenopus laevis gastrulation: combined roles for integrin alpha(5)beta(1), fibronectin, and tissue geometry. 2002, Pubmed , Xenbase
Davis, Mechanisms controlling human endothelial lumen formation and tube assembly in three-dimensional extracellular matrices. 2007, Pubmed
Downs, Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope. 1993, Pubmed
Essner, Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut. 2005, Pubmed
Fischer, FGF8 acts as a right determinant during establishment of the left-right axis in the rabbit. 2002, Pubmed
Fässler, Consequences of lack of beta 1 integrin gene expression in mice. 1995, Pubmed
George, Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin. 1993, Pubmed
George, Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells. 1997, Pubmed
Georges-Labouesse, Mesodermal development in mouse embryos mutant for fibronectin. 1996, Pubmed
Giancotti, Positional control of cell fate through joint integrin/receptor protein kinase signaling. 2003, Pubmed
Goodrich, Conservation of the hedgehog/patched signaling pathway from flies to mice: induction of a mouse patched gene by Hedgehog. 1996, Pubmed
Goodrich, Altered neural cell fates and medulloblastoma in mouse patched mutants. 1997, Pubmed
Grande, Nodal signalling is involved in left-right asymmetry in snails. 2009, Pubmed
Henrique, Expression of a Delta homologue in prospective neurons in the chick. 1995, Pubmed , Xenbase
Hynes, Integrins: bidirectional, allosteric signaling machines. 2002, Pubmed
Jülich, Control of extracellular matrix assembly along tissue boundaries via Integrin and Eph/Ephrin signaling. 2009, Pubmed
Jülich, Integrinalpha5 and delta/notch signaling have complementary spatiotemporal requirements during zebrafish somitogenesis. 2005, Pubmed
Kinder, The organizer of the mouse gastrula is composed of a dynamic population of progenitor cells for the axial mesoderm. 2001, Pubmed
Klass, Control of extracellular matrix assembly by syndecan-2 proteoglycan. 2000, Pubmed
Kramer, Ectodermal syndecan-2 mediates left-right axis formation in migrating mesoderm as a cell-nonautonomous Vg1 cofactor. 2002, Pubmed , Xenbase
Kumar, Broad mesodermal and endodermal deletion of Nodal at postgastrulation stages results solely in left/right axial defects. 2008, Pubmed
Kwon, The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages. 2008, Pubmed
Lee, Morphogenesis of the node and notochord: the cellular basis for the establishment and maintenance of left-right asymmetry in the mouse. 2008, Pubmed
Lee, Left-right patterning in the mouse requires Epb4.1l5-dependent morphogenesis of the node and midline. 2010, Pubmed
Lee, The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo. 2007, Pubmed
Levin, A molecular pathway determining left-right asymmetry in chick embryogenesis. 1995, Pubmed
Lohr, Maintenance of asymmetric nodal expression in Xenopus laevis. 1998, Pubmed , Xenbase
Marjoram, Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus. 2011, Pubmed , Xenbase
Marsden, Regulation of cell polarity, radial intercalation and epiboly in Xenopus: novel roles for integrin and fibronectin. 2001, Pubmed , Xenbase
Marsden, Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus. 2003, Pubmed , Xenbase
Massagué, TGF-beta signal transduction. 1998, Pubmed
Meno, Diffusion of nodal signaling activity in the absence of the feedback inhibitor Lefty2. 2001, Pubmed
Meno, lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal. 1998, Pubmed
Meyers, Differences in left-right axis pathways in mouse and chick: functions of FGF8 and SHH. 1999, Pubmed
Mine, BMP antagonism is required in both the node and lateral plate mesoderm for mammalian left-right axis establishment. 2008, Pubmed
Mittal, Fibronectin and integrin alpha 5 play essential roles in the development of the cardiac neural crest. 2010, Pubmed
Miyamoto, Integrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors. 1996, Pubmed
Nakamura, Generation of robust left-right asymmetry in the mouse embryo requires a self-enhancement and lateral-inhibition system. 2006, Pubmed
Nakaya, Wnt3a links left-right determination with segmentation and anteroposterior axis elongation. 2005, Pubmed
Neugebauer, FGF signalling during embryo development regulates cilia length in diverse epithelia. 2009, Pubmed , Xenbase
Nonaka, Determination of left-right patterning of the mouse embryo by artificial nodal flow. 2002, Pubmed
Norris, The Foxh1-dependent autoregulatory enhancer controls the level of Nodal signals in the mouse embryo. 2002, Pubmed
Oh, The signaling pathway mediated by the type IIB activin receptor controls axial patterning and lateral asymmetry in the mouse. 1997, Pubmed , Xenbase
Ohashi, Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein. 1999, Pubmed
Ohi, Anteriorward shifting of asymmetric Xnr1 expression and contralateral communication in left-right specification in Xenopus. 2007, Pubmed , Xenbase
Oki, Reversal of left-right asymmetry induced by aberrant Nodal signaling in the node of mouse embryos. 2009, Pubmed
Ramsdell, Left-right asymmetry and congenital cardiac defects: getting to the heart of the matter in vertebrate left-right axis determination. 2005, Pubmed
Ribes, Distinct Sonic Hedgehog signaling dynamics specify floor plate and ventral neuronal progenitors in the vertebrate neural tube. 2010, Pubmed
Saijoh, Distinct transcriptional regulatory mechanisms underlie left-right asymmetric expression of lefty-1 and lefty-2. 1999, Pubmed
Saijoh, Left-right asymmetric expression of lefty2 and nodal is induced by a signaling pathway that includes the transcription factor FAST2. 2000, Pubmed , Xenbase
Sakuma, Inhibition of Nodal signalling by Lefty mediated through interaction with common receptors and efficient diffusion. 2002, Pubmed , Xenbase
Saoncella, Syndecan-4 signals cooperatively with integrins in a Rho-dependent manner in the assembly of focal adhesions and actin stress fibers. 1999, Pubmed
Shiratori, The left-right axis in the mouse: from origin to morphology. 2006, Pubmed
Sulik, Morphogenesis of the murine node and notochordal plate. 1994, Pubmed
Takahashi, The RGD motif in fibronectin is essential for development but dispensable for fibril assembly. 2007, Pubmed
Trinh, Fibronectin regulates epithelial organization during myocardial migration in zebrafish. 2004, Pubmed
Tsukui, Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1. 1999, Pubmed
Yamamoto, The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse. 2001, Pubmed
Yamamoto, Nodal signaling induces the midline barrier by activating Nodal expression in the lateral plate. 2003, Pubmed
Yan, Conserved requirement for EGF-CFC genes in vertebrate left-right axis formation. 1999, Pubmed
Yang, Embryonic mesodermal defects in alpha 5 integrin-deficient mice. 1993, Pubmed
Yang, Cell adhesion events mediated by alpha 4 integrins are essential in placental and cardiac development. 1995, Pubmed
Yang, Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins. 1996, Pubmed
Yang, Overlapping and independent functions of fibronectin receptor integrins in early mesodermal development. 1999, Pubmed
Yelon, Cardiac patterning and morphogenesis in zebrafish. 2001, Pubmed
Yoshioka, Pitx2, a bicoid-type homeobox gene, is involved in a lefty-signaling pathway in determination of left-right asymmetry. 1998, Pubmed
Yost, Regulation of vertebrate left-right asymmetries by extracellular matrix. 1992, Pubmed , Xenbase
Zovein, Beta1 integrin establishes endothelial cell polarity and arteriolar lumen formation via a Par3-dependent mechanism. 2010, Pubmed
van der Flier, Function and interactions of integrins. 2001, Pubmed