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XB-ART-43047
PLoS One March 2, 2011; 6 (3): e18010.

XMeis3 is necessary for mesodermal Hox gene expression and function.



Abstract
Hox transcription factors provide positional information during patterning of the anteroposterior axis. Hox transcription factors can co-operatively bind with PBC-class co-factors, enhancing specificity and affinity for their appropriate binding sites. The nuclear localisation of these co-factors is regulated by the Meis-class of homeodomain proteins. During development of the zebrafish hindbrain, Meis3 has previously been shown to synergise with Hoxb1 in the autoregulation of Hoxb1. In Xenopus XMeis3 posteriorises the embryo upon ectopic expression. Recently, an early temporally collinear expression sequence of Hox genes was detected in Xenopus gastrula mesoderm (see intro. P3). There is evidence that this sequence sets up the embryo''s later axial Hox expression pattern by time-space translation. We investigated whether XMeis3 is involved in regulation of this early mesodermal Hox gene expression. Here, we present evidence that XMeis3 is necessary for expression of Hoxd1, Hoxb4 and Hoxc6 in mesoderm during gastrulation. In addition, we show that XMeis3 function is necessary for the progression of gastrulation. Finally, we present evidence for synergy between XMeis3 and Hoxd1 in Hoxd1 autoregulation in mesoderm during gastrulation.

PubMed ID: 21464931
PMC ID: PMC3065463
Article link: PLoS One

Genes referenced: cdx4 frzb2 hoxb1 hoxb4 hoxc6 hoxd1 meis3 tbxt
Morpholinos: meis3 MO2


Article Images: [+] show captions
References:
Abu-Shaar, 1999, Pubmed [+]


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