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XB-ART-43166
Dev Dyn. June 1, 2011; 240 (6): 1430-9.

Rab3d is required for Xenopus anterior neurulation by regulating Noggin secretion.



Abstract
Rab3d is a member of the Ras-related small GTPase family of secretory Rab, Rab3. In this study, we showed that Xenopus Rab3d is expressed specifically in the anterior border of the neural plate when the neural plate converges and folds to initiate neural tube formation. Morpholino-mediated knockdown of Rab3d resulted in neurulation defects both in neural plate convergence and folding. Interestingly, perturbation of BMP signaling rescued neurulation defects of Rab3d morphants, suggesting that Rab3d inhibits BMP signaling during neurulation. By secretion assay in the Xenopus animal cap, we found that Rab3d specifically regulates secretion of a BMP antagonist, Noggin, but not Chordin and Wnts. We also found that Rab3d is co-localized with Noggin and that this interaction is dependent on the GTP/GDP cycle of Rab3d. Collectively, these findings suggest that Rab3d-mediated secretion regulation of a BMP antagonist, Noggin, is one of the mechanisms of BMP antagonism during Xenopus anterior neurulation.

PubMed ID: 21520330
Article link: Dev Dyn.

Genes referenced: actl6a actn1 chrd.1 egr2 msx1 myc nog otx2 rab3d smad1 sox2 wnt5a wnt8a

Antibodies referenced: Smad1 Ab8
Morpholinos referenced: rab3d MO1


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