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XB-ART-43201
Proc Natl Acad Sci U S A. May 17, 2011; 108 (20): 8281-6.

Posttranscriptional activation of gene expression in Xenopus laevis oocytes by microRNA-protein complexes (microRNPs).

Mortensen RD , Serra M , Steitz JA , Vasudevan S .


Abstract
MicroRNA-protein complexes (microRNPs) can activate translation of target reporters and specific mRNAs in quiescent (i.e., G0) mammalian cell lines. Induced quiescent cells, like folliculated immature oocytes, have high levels of cAMP that activate protein kinase AII (PKAII) to maintain G0 and immature states. We report microRNA-mediated up-regulated expression of reporters in immature Xenopus laevis oocytes, dependent on Xenopus AGO or human AGO2 and on FXR1, as in mammalian cells. Importantly, we find that maintenance of cAMP levels and downstream PKAII signaling are required for microRNA-mediated up-regulated expression in oocytes. We identify an important, endogenous cell state regulator, Myt1 kinase, as a natural target of microRNA-mediated up-regulation in response to xlmiR16, ensuring maintenance of oocyte immaturity. Our data reveal the physiological relevance of cAMP/PKAII-controlled posttranscriptional gene expression activation by microRNAs in maintenance of the immature oocyte state.

PubMed ID: 21536868
PMC ID: PMC3100953
Article link: Proc Natl Acad Sci U S A.
Grant support: CA16038 NCI NIH HHS , GM26154 NIGMS NIH HHSHoward Hughes Medical Institute , CA16038 NCI NIH HHSHoward Hughes Medical Institute , P01 CA016038 NCI NIH HHS , R01 GM026154 NIGMS NIH HHS , GM26154 NIGMS NIH HHS

Genes referenced: ago2 cdk1 ctrl fxr1 igf2bp3 myt1 pak1 ren


References:
Armisen, 2009, Pubmed, Xenbase[+]


Article Images: [+] show captions

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