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XB-ART-43209
Neural Dev April 30, 2011; 6 19.

EBF factors drive expression of multiple classes of target genes governing neuronal development.



Abstract
Early B cell factor (EBF) family members are transcription factors known to have important roles in several aspects of vertebrate neurogenesis, including commitment, migration and differentiation. Knowledge of how EBF family members contribute to neurogenesis is limited by a lack of detailed understanding of genes that are transcriptionally regulated by these factors. We performed a microarray screen in Xenopus animal caps to search for targets of EBF transcriptional activity, and identified candidate targets with multiple roles, including transcription factors of several classes. We determined that, among the most upregulated candidate genes with expected neuronal functions, most require EBF activity for some or all of their expression, and most have overlapping expression with ebf genes. We also found that the candidate target genes that had the most strongly overlapping expression patterns with ebf genes were predicted to be direct transcriptional targets of EBF transcriptional activity. The identification of candidate targets that are transcription factor genes, including nscl-1, emx1 and aml1, improves our understanding of how EBF proteins participate in the hierarchy of transcription control during neuronal development, and suggests novel mechanisms by which EBF activity promotes migration and differentiation. Other candidate targets, including pcdh8 and kcnk5, expand our knowledge of the types of terminal differentiated neuronal functions that EBF proteins regulate.

PubMed ID: 21529371
PMC ID: PMC3113313
Article link: Neural Dev
Grant support: [+]
Genes referenced: ebf2 ebf3 emx1 emx1l en2 gal.2 greb1 hist1h4d hoxd10 inhbb kcnk5 nefm neurod1 nhlh1 nog nr2f2 pcdh8 pcdh8.2 prph runx1 sox2 wnt3a
Morpholinos: ebf2 MO1 ebf3 MO1

GEO Series:
   GSE25734: NCBI
   GSE27084: NCBI


Article Images: [+] show captions
References:
Akerblad, 2005, Pubmed [+]


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