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XB-ART-43221
Development June 1, 2011; 138 (11): 2369-78.

Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis.

Bugner V , Tecza A , Gessert S , Kühl M .


Abstract
The Xenopus oocyte possesses a large maternal store of ribosomes, thereby uncoupling early development from the de novo ribosome biosynthesis required for cell growth. Brix domain-containing proteins, such as Peter Pan (PPan), are essential for eukaryotic ribosome biogenesis. In this study, we demonstrate that PPan is expressed maternally as well as in the eye and cranial neural crest cells (NCCs) during early Xenopus laevis development. Depletion of PPan and interference with rRNA processing using antisense morpholino oligonucleotides resulted in eye and cranial cartilage malformations. Loss of PPan, but not interference with rRNA processing, led to an early downregulation of specific marker genes of the eye, including Rx1 and Pax6, and of NCCs, such as Twist, Slug and FoxD3. We found that PPan protein is localized in the nucleoli and mitochondria and that loss of PPan results in increased apoptosis. These findings indicate a novel function of PPan that is independent of its role in ribosome biogenesis.

PubMed ID: 21558383
Article link: Development

Genes referenced: acss2.2 bcl2 brix1 emx1 emx1.2 en2 foxd3 myc nog otx2 pax6 ppan psmd6 rax snai2 sox3 tbx2 tp53 twist1 ubtf wnt4
Morpholinos referenced: fzd3 MO1 myc MO2 ppan MO1 ppan MO2 tp53 MO1 wnt4 MO1



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