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XB-ART-43265
Dev Dyn July 1, 2011; 240 (7): 1705-15.

Xenopus laevis insulin receptor substrate IRS-1 is important for eye development.

Bugner V , Aurhammer T , Kühl M .


Abstract
Extracellular signal transduction into cells through ligand-activated receptor tyrosine kinases, such as insulin-like growth factor-1 (IGF-1) receptor (IGF-1R) and insulin receptor (IR) is required for normal embryonic growth and development. The major mediators of IR and IGF-1R are adaptor proteins of the insulin receptor substrate family, the best characterized member of which is IRS-1. Insulin receptor substrate IRS-1 has been shown to influence cell and body size and to interfere with differentiation. We have isolated IRS-1 from Xenopus laevis embryos and analyzed for the first time its spatial and temporal expression pattern during embryogenesis. We found that Xenopus IRS-1 is expressed maternally and constantly during embryogenesis. It is predominantly found in neural tissue at different stages. Furthermore, knock down of IRS-1 in neural tissue by specific antisense morpholino oligonucleotides (MO) resulted in abnormal eye formation accompanied by reduction of the eye-specific marker genes Rx1 and Pax6 and a decreased cell proliferation.

PubMed ID: 21574211
Article link: Dev Dyn

Genes referenced: egr2 emx1 emx1l en2 foxe3 igf1 igf1r ins insr irs1 nppa otx2 pax6 rax sox3
Morpholinos: irs1 MO1 irs1 MO2


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