XB-ART-43344Genes Dev. June 1, 2011; 25 (11): 1105-8.
RNAi in Xenopus: look before you leap.
RNAi has revolutionized reverse genetics; however, RNAi is not necessarily ubiquitous or constitutive. Lund and colleagues (pp. 1121-1131) show that microRNA (miRNA) effector Argonautes (Agos) are limiting and easily saturated during early Xenopus embryogenesis. Moreover, this stage is devoid of slicing capacity. Supplementation of Ago proteins rescued endogenous miRNA activity in the presence of exogenous siRNAs, and, excitingly, ectopic Ago2 could now support RNAi in Xenopus. These observations may potentially facilitate RNAi in other recalcitrant model organisms.
PubMed ID: 21632820
PMC ID: PMC3110948
Article link: Genes Dev.
Grant support: R01GM083300 NIGMS NIH HHS , R01 GM083300 NIGMS NIH HHS , R01GM083300 NIGMS NIH HHS
Genes referenced: ago2