XB-ART-43522Dev Biol September 15, 2011; 357 (2): 478-91.
An essential role for transcription before the MBT in Xenopus laevis.
PubMed ID: 21741375
PMC ID: PMC3164747
Article link: Dev Biol
Grant support: 1R01GM076621 NIGMS NIH HHS , R01 GM076621-01A1 NIGMS NIH HHS , R01 GM076621-02 NIGMS NIH HHS , R01 GM076621-03 NIGMS NIH HHS , R01 GM076621-04 NIGMS NIH HHS , R01 GM093217-02 NIGMS NIH HHS , R01GM093217 NIGMS NIH HHS , T32-GM07229 NIGMS NIH HHS , R01 GM093217 NIGMS NIH HHS , R01 GM076621 NIGMS NIH HHS , T32 GM007229 NIGMS NIH HHS
Genes referenced: chrd.1 foxi1 gdf3 gsc mixer nodal nodal1 nodal5.2 nodal6 smad2 sox17a tbxt vegt
Morpholinos referenced: ctnnb1 MO1 nodal5.2 MO1 nodal5.2 MO2 nodal6 MO1 nodal6 MO2
Article Images: [+] show captions
|Fig. 4. preMBT Nodal signaling is required for mesendoderm induction. (A) Embryos were treated with control medium (nt) or SB5 (200 μM) from the 4-cell stage to stage 10 and harvested for Western blot for P-Smad2/3 or Smad2/3. (B) Embryos were treated with SB5 from the indicated stages until the onset of gastrulation and cultured until stage 40. (C) Embryos were treated with SB5 beginning at the indicated stages, cultured until stage 10, and analyzed by in situ hybridization (ISH) for mesendodermal gene expression (left). control indicates DMSO (vehicle) only. Embryos were also harvested at early gastrula stage (stage 10) for qRT-PCR for the indicated mesendodermal markers and for the ectodermal marker foxi1e (right panel). All samples were normalized to expression levels in untreated embryos at stage 10 (control). control indicates DMSO; B5indicates treatment from 4-cell until gastrula (stage 10). (D) SB5 was added at the 4-cell stage; embryos were washed at the indicated stages, transferred to control medium, and analyzed by ISH for mesendodermal gene expression at stage 10, as in (C). Histogram on right shows qRT-PCR for mesendodermal and ectodermal markers, as in (C). Note that the control and B5(4-cell until stage 10) qRT-PCR data in panel D are identical to those shown in panel C, as qRT-PCR for both groups was performed at the same time.|
|Fig. 7. Nodal signaling contributes to xnr5/6 expression. (A) SB5 (200 μM) was added at the 4-cell stage and embryos were fixed for ISH at MBT (stage 8.5). Whole-embryos were photographed in vegetal views, dorsal to the top (vegetal view). Embryos bisected along the dorsal/ventral axis prior to ISH are shown with dorsal side to the right (lateral view). The frequency of the expression pattern for each group is indicated in the lower right corner. DMSO was used as a control. (B) To inhibit Nodal signaling, the dominant-negative Nodal receptor (DN-ALK4) was injected (1 ng), along with fluorescent dextran (insets, rust-colored staining), into the 2 right-hand blastomeres of 4-cell embryos. Embryos were fixed at the indicated times and analyzed by ISH. Fluorescent dextran injection alone is shown as control. The number of cells expressing xnr5 or xnr6 in injected clones was counted and significance was determined using the Kruskalallis test. (C) 8-cell embryos were injected in one animal cell with GR-tSmad2 (400 pg) and fluorescent dextran. GR-tSmad2 was activated by addition of dex for a two-hour window beginning at the indicated stages. Embryos were fixed at the end of the window and analyzed by ISH.|
|Fig. 8. xnr5 and xnr6 can activate Smad2 and regulate their own expression before the MBT (A) 8-cell embryos were injected in one animal cell with fluorescent dextran alone (control) or with xnr1 (50 pg), xnr5 (20 pg), or xnr6 (20 pg) mRNAs. Whole mount ISH for xnr5, xnr6, and chd was performed on embryos fixed at the indicated stages. (B) Embryos from A were analyzed by qRT-PCR at stage 8 (preMBT). (C) 8-cell embryos were injected in one animal cell with GFP-Smad2 (200 pg) alone or with xnr5 mRNA (50 pg). Embryos were fixed at the 512-cell stage, processed for anti-GFP immunostaining, and sectioned for fluorescence imaging. DAPI was used to reveal nuclei. The graph shows the percent of DAPI positive nuclei that also contain GFP-Smad2. (D) Embryos injected as in C were fixed at various stages as indicated and stained for xnr6 expression. ui, uninjected.|
|Fig. 6. Smad2 rescues mesendodermal gene expression when activated before but not after the MBT. Embryos were injected with a hormone inducible, activated form of Smad2 (GRtSmad2) at the 2-cell stage (along with FLDx as a lineage tracer) and then treated with SB5 beginning at the 4-cell stage. GR-tSmad2 was activated by addition of dexamethasone (dex) before the MBT (stage 7) or after the MBT (stage 9) and embryos were cultured until the onset of gastrulation and then collected for analysis of mesendodermal gene expression by ISH (A) or qRT-PCR (B), as described in Fig. 4. (In panel A, the insets show FLDx immunostaining to reveal the progeny of injected blastoemeres and the numbers in the upper left indicate the frequency of the displayed phenotype/expression pattern). Error bars represent standard deviation for a representative experiment.|