XB-ART-43524
Neuropharmacology
2011 Jan 01;615-6:918-23. doi: 10.1016/j.neuropharm.2011.06.020.
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TREK-1 isoforms generated by alternative translation initiation display different susceptibility to the antidepressant fluoxetine.
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Two-pore-domain K(+) (K(2)P) channels are highly expressed in neurons and cardiac myocytes. In this study we investigated the potency of the antidepressant fluoxetine to inhibit brain and cardiac K(2)P channels, TREK-1, TASK-1 and THIK-1. Maximal sensitivity was detected for TREK-1, which was inhibited by 77% when expressed in HEK-293 cells and Xenopus oocytes. Alternative translation initiation (ATI) generates two different protein products from a single transcript of TREK-1. Electrophysiological analysis of two polypeptides engineered by mutagenesis (TREK-1[M53I], TREK-1[ΔN52]) revealed reduced current amplitude and K(+) selectivity of the truncated TREK-1 isoform. The sensitivity of TREK-1[ΔN52] to fluoxetine decreased by 70%, indicating that the first 52 amino acids are essential for TREK-1 sensitivity to this drug.
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Species referenced: Xenopus laevis
Genes referenced: kcnk2