Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-43550
Dev Dyn. August 1, 2011; 240 (8): 1889-904.

V-ATPase-dependent ectodermal voltage and pH regionalization are required for craniofacial morphogenesis.

Vandenberg LN , Morrie RD , Adams DS .


Abstract
Using voltage and pH reporter dyes, we have discovered a never-before-seen regionalization of the Xenopus ectoderm, with cell subpopulations delimited by different membrane voltage and pH. We distinguished three courses of bioelectrical activity. Course I is a wave of hyperpolarization that travels across the gastrula. Course II comprises the appearance of patterns that match shape changes and gene expression domains of the developing face; hyperpolarization marks folding epithelium and both hyperpolarized and depolarized regions overlap domains of head patterning genes. In Course III, localized regions of hyperpolarization form at various positions, expand, and disappear. Inhibiting H(+) -transport by the H(+) -V-ATPase causes abnormalities in: (1) the morphology of craniofacial structures; (2) Course II voltage patterns; and (3) patterns of sox9, pax8, slug, mitf, xfz3, otx2, and pax6. We conclude that this bioelectric signal has a role in development of the face. Thus, it exemplifies an important, under-studied mechanism of developmental regulation.

PubMed ID: 21761475
Article link: Dev Dyn.
Grant support: 1F32GM087107-01 NIGMS NIH HHS , K22-DE016633 NIDCR NIH HHS

Genes referenced: atp6v0c fzd3 gal.2 mitf nodal1 otx2 pax6 pax8 slc9a3 snai2 sox9
Antibodies referenced: Atp6v0c Ab1
Morpholinos referenced:
Article Images: [+] show captions

My Xenbase: [ Log-in / Register ]
version: [3.3]


Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556