XB-ART-43655
J Am Soc Nephrol
2011 Sep 01;229:1654-64. doi: 10.1681/ASN.2010101086.
Show Gene links
Show Anatomy links
Pronephric tubulogenesis requires Daam1-mediated planar cell polarity signaling.
???displayArticle.abstract???
Canonical β-catenin-mediated Wnt signaling is essential for the induction of nephron development. Noncanonical Wnt/planar cell polarity (PCP) pathways contribute to processes such as cell polarization and cytoskeletal modulation in several tissues. Although PCP components likely establish the plane of polarization in kidney tubulogenesis, whether PCP effectors directly modulate the actin cytoskeleton in tubulogenesis is unknown. Here, we investigated the roles of Wnt PCP components in cytoskeletal assembly during kidney tubule morphogenesis in Xenopus laevis and zebrafish. We found that during tubulogenesis, the developing pronephric anlagen expresses Daam1 and its interacting Rho-GEF (WGEF), which compose one PCP/noncanonical Wnt pathway branch. Knockdown of Daam1 resulted in reduced expression of late pronephric epithelial markers with no apparent effect upon early markers of patterning and determination. Inhibiting various points in the Daam1 signaling pathway significantly reduced pronephric tubulogenesis. These data indicate that pronephric tubulogenesis requires the Daam1/WGEF/Rho PCP pathway.
???displayArticle.pubmedLink??? 21804089
???displayArticle.pmcLink??? PMC3171937
???displayArticle.link??? J Am Soc Nephrol
???displayArticle.grants??? [+]
CA-16672 NCI NIH HHS , DK082145 NIDDK NIH HHS , HD07325 NICHD NIH HHS , R01 GM052112 NIGMS NIH HHS , R01 GM078172-07 NIGMS NIH HHS , F32 DK082145 NIDDK NIH HHS , T32 HD007325 NICHD NIH HHS , R01 GM078172 NIGMS NIH HHS , P30 CA016672 NCI NIH HHS
Species referenced: Xenopus laevis
Genes referenced: actl6a arhgef19 clock ctnnb1 daam1 fat4 invs rho rho.2 vangl2 wnt11 wnt11b wnt4 wnt5a wnt6 wnt7b wnt8a wnt8b wnt9a wnt9b
???displayArticle.antibodies??? H3f3a Ab9 Kidney Ab1 Kidney Ab2 Somite Ab1
???displayArticle.morpholinos??? arhgef19 MO2 daam1 MO1
References [+] :
Agrawal,
The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1.
2009, Pubmed,
Xenbase
Agrawal, The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1. 2009, Pubmed , Xenbase
Baarlink, SnapShot: Formins. 2010, Pubmed
Barnett, Neural induction and patterning by fibroblast growth factor, notochord and somite tissue in Xenopus. 1998, Pubmed , Xenbase
Borovina, Vangl2 directs the posterior tilting and asymmetric localization of motile primary cilia. 2010, Pubmed
Bouchard, Nephric lineage specification by Pax2 and Pax8. 2002, Pubmed
Brade, The role of Wnt signalling in cardiac development and tissue remodelling in the mature heart. 2006, Pubmed
Brändli, Towards a molecular anatomy of the Xenopus pronephric kidney. 1999, Pubmed , Xenbase
Cardoso, Regulation of early lung morphogenesis: questions, facts and controversies. 2006, Pubmed
Carreira-Barbosa, Prickle 1 regulates cell movements during gastrulation and neuronal migration in zebrafish. 2003, Pubmed
Carroll, Synergism between Pax-8 and lim-1 in embryonic kidney development. 1999, Pubmed , Xenbase
Carroll, Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. 2005, Pubmed , Xenbase
Cervantes, Wnt5a is essential for intestinal elongation in mice. 2009, Pubmed
Cha, Wnt5a and Wnt11 interact in a maternal Dkk1-regulated fashion to activate both canonical and non-canonical signaling in Xenopus axis formation. 2008, Pubmed , Xenbase
Chan, Growing kidney in the frog. 2006, Pubmed , Xenbase
Chhabra, The many faces of actin: matching assembly factors with cellular structures. 2007, Pubmed
Choi, Xenopus Cdc42 regulates convergent extension movements during gastrulation through Wnt/Ca2+ signaling pathway. 2002, Pubmed , Xenbase
Cohen, Wnt signaling: an essential regulator of cardiovascular differentiation, morphogenesis and progenitor self-renewal. 2008, Pubmed
Costantini, Renal branching morphogenesis: concepts, questions, and recent advances. 2006, Pubmed
Curtin, Mutation of Celsr1 disrupts planar polarity of inner ear hair cells and causes severe neural tube defects in the mouse. 2003, Pubmed
Dale, The emerging role of Wnt/PCP signaling in organ formation. 2009, Pubmed
De Langhe, Dickkopf-1 (DKK1) reveals that fibronectin is a major target of Wnt signaling in branching morphogenesis of the mouse embryonic lung. 2005, Pubmed
Dressler, The cellular basis of kidney development. 2006, Pubmed , Xenbase
Drummond, Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function. 1998, Pubmed
Eid, Xenopus Na,K-ATPase: primary sequence of the beta2 subunit and in situ localization of alpha1, beta1, and gamma expression during pronephric kidney development. 2001, Pubmed , Xenbase
Etheridge, Murine dishevelled 3 functions in redundant pathways with dishevelled 1 and 2 in normal cardiac outflow tract, cochlea, and neural tube development. 2008, Pubmed
Faix, Staying in shape with formins. 2006, Pubmed
Fang, Vertebrate development requires ARVCF and p120 catenins and their interplay with RhoA and Rac. 2004, Pubmed , Xenbase
Freter, Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential. 2008, Pubmed
Gerth, Nephrin expression and three-dimensional morphogenesis of the Xenopus pronephric glomus. 2005, Pubmed , Xenbase
Goto, The planar cell polarity gene strabismus regulates convergence and extension and neural fold closure in Xenopus. 2002, Pubmed , Xenbase
Gu, Xenopus delta-catenin is essential in early embryogenesis and is functionally linked to cadherins and small GTPases. 2009, Pubmed , Xenbase
Habas, Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel Formin homology protein Daam1. 2001, Pubmed , Xenbase
Habas, Dishevelled and Wnt signaling: is the nucleus the final frontier? 2005, Pubmed
Habas, Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation. 2003, Pubmed , Xenbase
Harland, In situ hybridization: an improved whole-mount method for Xenopus embryos. 1991, Pubmed , Xenbase
Heisenberg, Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation. 2000, Pubmed
Hukriede, Making a tubule the noncanonical way. 2011, Pubmed , Xenbase
Iglesias, Canonical WNT signaling during kidney development. 2007, Pubmed
Imbert, Delta N89 beta-catenin induces precocious development, differentiation, and neoplasia in mammary gland. 2001, Pubmed
Itäranta, Wnt-6 is expressed in the ureter bud and induces kidney tubule development in vitro. 2002, Pubmed , Xenbase
Jayasena, Notch signaling augments the canonical Wnt pathway to specify the size of the otic placode. 2008, Pubmed
Jenny, Planar cell polarity signaling: a common mechanism for cellular polarization. 2006, Pubmed
Jones, Xenopus: a prince among models for pronephric kidney development. 2005, Pubmed , Xenbase
Karner, Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis. 2009, Pubmed , Xenbase
Khadka, Non-redundant roles for Profilin2 and Profilin1 during vertebrate gastrulation. 2009, Pubmed , Xenbase
Kim, Isolation and characterization of XKaiso, a transcriptional repressor that associates with the catenin Xp120(ctn) in Xenopus laevis. 2002, Pubmed , Xenbase
Kim, JNK and ROKalpha function in the noncanonical Wnt/RhoA signaling pathway to regulate Xenopus convergent extension movements. 2005, Pubmed , Xenbase
Kimmel, Stages of embryonic development of the zebrafish. 1995, Pubmed , Xenbase
Kintner, Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration. , Pubmed , Xenbase
Kishimoto, Cystic kidney gene seahorse regulates cilia-mediated processes and Wnt pathways. 2008, Pubmed
Kispert, Wnt-4 is a mesenchymal signal for epithelial transformation of metanephric mesenchyme in the developing kidney. 1998, Pubmed
Komiya, Wnt signal transduction pathways. 2008, Pubmed
Li, Wnt5a participates in distal lung morphogenesis. 2002, Pubmed
Lin, Induction of ureter branching as a response to Wnt-2b signaling during early kidney organogenesis. 2001, Pubmed
Liu, Mechanism of activation of the Formin protein Daam1. 2008, Pubmed , Xenbase
Lyons, Requirement of Wnt/beta-catenin signaling in pronephric kidney development. 2009, Pubmed , Xenbase
Lyons, Wnt-4 activates the canonical beta-catenin-mediated Wnt pathway and binds Frizzled-6 CRD: functional implications of Wnt/beta-catenin activity in kidney epithelial cells. 2004, Pubmed , Xenbase
Majumdar, Wnt11 and Ret/Gdnf pathways cooperate in regulating ureteric branching during metanephric kidney development. 2003, Pubmed
Matakatsu, Interactions between Fat and Dachsous and the regulation of planar cell polarity in the Drosophila wing. 2004, Pubmed
Mauch, Signals from trunk paraxial mesoderm induce pronephros formation in chick intermediate mesoderm. 2000, Pubmed
Mayor, Induction of the prospective neural crest of Xenopus. 1995, Pubmed , Xenbase
McNeill, Planar cell polarity and the kidney. 2009, Pubmed
Merkel, Molecular regulation of kidney development: is the answer blowing in the Wnt? 2007, Pubmed
Miller, Wnt to build a tube: contributions of Wnt signaling to epithelial tubulogenesis. 2010, Pubmed , Xenbase
Montcouquiol, Asymmetric localization of Vangl2 and Fz3 indicate novel mechanisms for planar cell polarity in mammals. 2006, Pubmed
Montcouquiol, Identification of Vangl2 and Scrb1 as planar polarity genes in mammals. 2003, Pubmed
Moody, Fates of the blastomeres of the 16-cell stage Xenopus embryo. 1987, Pubmed , Xenbase
Moody, Fates of the blastomeres of the 32-cell-stage Xenopus embryo. 1987, Pubmed , Xenbase
Nakaya, Identification and comparative expression analyses of Daam genes in mouse and Xenopus. 2004, Pubmed , Xenbase
Ohyama, Wnt signals mediate a fate decision between otic placode and epidermis. 2006, Pubmed
Okubo, Hyperactive Wnt signaling changes the developmental potential of embryonic lung endoderm. 2004, Pubmed
Park, Frodo links Dishevelled to the p120-catenin/Kaiso pathway: distinct catenin subfamilies promote Wnt signals. 2006, Pubmed , Xenbase
Park, Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells. 2008, Pubmed , Xenbase
Park, Wnt/beta-catenin signaling regulates nephron induction during mouse kidney development. 2007, Pubmed
Qian, Wnt5a functions in planar cell polarity regulation in mice. 2007, Pubmed , Xenbase
Rajagopal, Wnt7b stimulates embryonic lung growth by coordinately increasing the replication of epithelium and mesenchyme. 2008, Pubmed
Riccomagno, Wnt-dependent regulation of inner ear morphogenesis is balanced by the opposing and supporting roles of Shh. 2005, Pubmed
Robinson, Side-branching in the mammary gland: the progesterone-Wnt connection. 2000, Pubmed
Roszko, Regulation of convergence and extension movements during vertebrate gastrulation by the Wnt/PCP pathway. 2009, Pubmed
Rubin, Intestinal morphogenesis. 2007, Pubmed , Xenbase
Saburi, Loss of Fat4 disrupts PCP signaling and oriented cell division and leads to cystic kidney disease. 2008, Pubmed
Saka, Spatial and temporal patterns of cell division during early Xenopus embryogenesis. 2001, Pubmed , Xenbase
Sato, Profilin is an effector for Daam1 in non-canonical Wnt signaling and is required for vertebrate gastrulation. 2006, Pubmed , Xenbase
Saulnier, Essential function of Wnt-4 for tubulogenesis in the Xenopus pronephric kidney. 2002, Pubmed , Xenbase
Schedl, Renal abnormalities and their developmental origin. 2007, Pubmed
Schmidt-Ott, beta-catenin/TCF/Lef controls a differentiation-associated transcriptional program in renal epithelial progenitors. 2007, Pubmed
Schmidt-Ott, WNT/beta-catenin signaling in nephron progenitors and their epithelial progeny. 2008, Pubmed
Schneider, Wnt antagonism initiates cardiogenesis in Xenopus laevis. 2001, Pubmed , Xenbase
Schreiber, Remodeling of the intestine during metamorphosis of Xenopus laevis. 2005, Pubmed , Xenbase
Seifert, Frizzled/PCP signalling: a conserved mechanism regulating cell polarity and directed motility. 2007, Pubmed
Seufert, Developmental basis of pronephric defects in Xenopus body plan phenotypes. 1999, Pubmed , Xenbase
Shu, Wnt7b regulates mesenchymal proliferation and vascular development in the lung. 2002, Pubmed
Simons, Inversin, the gene product mutated in nephronophthisis type II, functions as a molecular switch between Wnt signaling pathways. 2005, Pubmed , Xenbase
Skouloudaki, Scribble participates in Hippo signaling and is required for normal zebrafish pronephros development. 2009, Pubmed
Stark, Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4. 1994, Pubmed
Stewart, Dual fluorescent protein reporters for studying cell behaviors in vivo. 2009, Pubmed
Strehlow, The fates of the blastomeres of the 16-cell zebrafish embryo. 1994, Pubmed
Tada, Noncanonical Wnt/PCP signaling during vertebrate gastrulation. 2009, Pubmed , Xenbase
Taira, The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos. 1992, Pubmed , Xenbase
Takeyasu, Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump. 1988, Pubmed
Tanegashima, WGEF activates Rho in the Wnt-PCP pathway and controls convergent extension in Xenopus gastrulation. 2008, Pubmed , Xenbase
Tételin, Xenopus Wnt11b is identified as a potential pronephric inducer. 2010, Pubmed , Xenbase
Ueno, Planar cell polarity genes and neural tube closure. 2003, Pubmed , Xenbase
Veeman, Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. 2003, Pubmed
Verzi, Wnt signaling in gut organogenesis. 2008, Pubmed
Vize, Model systems for the study of kidney development: use of the pronephros in the analysis of organ induction and patterning. 1997, Pubmed , Xenbase
Vize, The chloride conductance channel ClC-K is a specific marker for the Xenopus pronephric distal tubule and duct. 2003, Pubmed , Xenbase
Vize, Development of the Xenopus pronephric system. 1995, Pubmed , Xenbase
Wallingford, Precocious expression of the Wilms' tumor gene xWT1 inhibits embryonic kidney development in Xenopus laevis. 1998, Pubmed , Xenbase
Wallingford, Vertebrate gastrulation: polarity genes control the matrix. 2005, Pubmed , Xenbase
Wallingford, The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity. 2005, Pubmed
Wang, Dishevelled genes mediate a conserved mammalian PCP pathway to regulate convergent extension during neurulation. 2006, Pubmed , Xenbase
Wang, Regulation of polarized extension and planar cell polarity in the cochlea by the vertebrate PCP pathway. 2005, Pubmed
Wang, The role of Frizzled3 and Frizzled6 in neural tube closure and in the planar polarity of inner-ear sensory hair cells. 2006, Pubmed
Wingert, The zebrafish pronephros: a model to study nephron segmentation. 2008, Pubmed , Xenbase
Yates, The PCP genes Celsr1 and Vangl2 are required for normal lung branching morphogenesis. 2010, Pubmed
Yu, A Wnt7b-dependent pathway regulates the orientation of epithelial cell division and establishes the cortico-medullary axis of the mammalian kidney. 2009, Pubmed
Zhou, Proximo-distal specialization of epithelial transport processes within the Xenopus pronephric kidney tubules. 2004, Pubmed , Xenbase
von Strandmann, Patterning the expression of a tissue-specific transcription factor in embryogenesis: HNF1 alpha gene activation during Xenopus development. 1997, Pubmed , Xenbase