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XB-ART-43682
PLoS One January 1, 2011; 6 (7): e21796.

The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression.

Lee SY , Yoon J , Lee HS , Hwang YS , Cha SW , Jeong CH , Kim JI , Park JB , Lee JY , Kim S , Park MJ , Dong Z , Kim J .


Abstract
BACKGROUND: Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS: We provide new evidence showing that overexpression of heterodimeric AP-1 comprised of c-jun and c-fos (AP-1(c-Jun/c-Fos)) induces the expression of BMP-antagonizing organizer genes (noggin, chordin and goosecoid) that were normally expressed by high dose of activin. AP-1(c-Jun/c-Fos) enhanced the promoter activities of organizer genes but reduced that of PV.1, a BMP4-response gene. A loss of function study clearly demonstrated that AP-1(c-Jun/c-Fos) is required for the activin-induced organizer and neural gene expression. Moreover, physical interaction of AP-1(c-Jun/c-Fos) and Smad3 cooperatively enhanced the transcriptional activity of goosecoid via direct binding on this promoter. Interestingly, Smad3 mutants at c-Jun binding site failed in regulation of organizer genes, indicating that these physical interactions are specifically necessary for the expression of organizer genes. CONCLUSIONS/SIGNIFICANCE: AP-1(c-Jun/c-Fos) plays a specific role in organizer gene expression in downstream of activin signal during early Xenopus embryogenesis.

PubMed ID: 21829441
PMC ID: PMC3146467
Article link: PLoS One
Grant support: [+]
Genes referenced: actl6a bmp4 chrd.1 fos gata2 gsc jun krt12.4 myc ncam1 nog odc1 smad3 tbxt ventx1.1
Morpholinos: jun MO2


Article Images: [+] show captions
References [+] :
Amaya, FGF signalling in the early specification of mesoderm in Xenopus. 1993, Pubmed, Xenbase


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