Due to necessary maintenance, Xenbase will be unavailable December 24-30, 2014. We apologize for the inconvenience.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-43683
Genes Dev. August 1, 2011; 25 (15): 1654-61.

HEB and E2A function as SMAD/FOXH1 cofactors.

Yoon SJ , Wills AE , Chuong E , Gupta R , Baker JC .


Abstract
Nodal signaling, mediated through SMAD transcription factors, is necessary for pluripotency maintenance and endoderm commitment. We identified a new motif, termed SMAD complex-associated (SCA), that is bound by SMAD2/3/4 and FOXH1 in human embryonic stem cells (hESCs) and derived endoderm. We demonstrate that two basic helix-loop-helix (bHLH) proteins-HEB and E2A-bind the SCA motif at regions overlapping SMAD2/3 and FOXH1. Furthermore, we show that HEB and E2A associate with SMAD2/3 and FOXH1, suggesting they form a complex at critical target regions. This association is biologically important, as E2A is critical for mesendoderm specification, gastrulation, and Nodal signal transduction in Xenopus tropicalis embryos. Taken together, E proteins are novel Nodal signaling cofactors that associate with SMAD2/3 and FOXH1 and are necessary for mesendoderm differentiation.

PubMed ID: 21828274
PMC ID: PMC3182016
Article link: Genes Dev.
Grant support: F32DK089643-01 NIDDK NIH HHS , T32 HG000044 NHGRI NIH HHS

Genes referenced: cer1 foxh1.2 gal.2 gapdh gsc lefty mixer nodal nodal1 odc1 smad2 smad3 smad4.1 sox17b.1 t tcf12 tcf3 trim9
Antibodies referenced:
Morpholinos referenced: tcf12 MO1 tcf12 MO2 tcf3 MO1
Article Images: [+] show captions

My Xenbase: [ Log-in / Register ]
version: [3.3.1]


Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556