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XB-ART-43793
Dev Biol October 1, 2011; 358 (1): 262-76.

The dual regulator Sufu integrates Hedgehog and Wnt signals in the early Xenopus embryo.

Min TH , Kriebel M , Hou S , Pera EM .


Abstract
Hedgehog (Hh) and Wnt proteins are important signals implicated in several aspects of embryonic development, including the early development of the central nervous system. We found that Xenopus Suppressor-of-fused (XSufu) affects neural induction and patterning by regulating the Hh/Gli and Wnt/β-catenin pathways. Microinjection of XSufu mRNA induced expansion of the epidermis at the expense of neural plate tissue and caused enlargement of the eyes. An antisense morpholino oligonucleotide against XSufu had the opposite effect. Interestingly, both gain- and loss-of-function experiments resulted in a posterior shift of brain markers, suggesting a biphasic effect of XSufu on anteroposterior patterning. XSufu blocked early Wnt/β-catenin signaling, as indicated by the suppression of XWnt8-induced secondary axis formation in mRNA-injected embryos, and activation of Wnt target genes in XSufu-MO-injected ectodermal explants. We show that XSufu binds to XGli1 and Xβ-catenin. In Xenopus embryos and mouse embryonic fibroblasts, Gli1 inhibits Wnt signaling under overexpression of β-catenin, whereas β-catenin stimulates Hh signaling under overexpression of Gli1. Notably, endogenous Sufu is critically involved in this crosstalk. The results suggest that XSufu may act as a common regulator of Hh and Wnt signaling and contribute to intertwining the two pathways.

PubMed ID: 21839734
Article link: Dev Biol

Genes referenced: ag1 chrd.1 egr2 en2 foxg1 gli1 h4c4 hoxc6 ihh krt12.4 myod1 nodal3.1 nodal3.2 nppa otx2 ptch1 rax rax2 shh sia1 snai1 snai2 sox2 sufu tubb2b wnt3a wnt8a
Morpholinos: sufu MO1


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