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XB-ART-44098
Nat Cell Biol April 1, 2011; 13 (4): 351-60.

Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.

Kim S , Zaghloul NA , Bubenshchikova E , Oh EC , Rankin S , Katsanis N , Obara T , Tsiokas L .


Abstract
The primary cilium is an antenna-like organelle that is dynamically regulated during the cell cycle. Ciliogenesis is initiated as cells enter quiescence, whereas resorption of the cilium precedes mitosis. The mechanisms coordinating ciliogenesis with the cell cycle are unknown. Here we identify the centrosomal protein Nde1 (nuclear distribution gene E homologue 1) as a negative regulator of ciliary length. Nde1 is expressed at high levels in mitosis, low levels in quiescence and localizes at the mother centriole, which nucleates the primary cilium. Cells depleted of Nde1 have longer cilia and a delay in cell cycle re-entry that correlates with ciliary length. Knockdown of Nde1 in zebrafish embryos results in increased ciliary length, suppression of cell division, reduction of the number of cells forming the Kupffer''s vesicle and left-right patterning defects. These data suggest that Nde1 is an integral component of a network coordinating ciliary length with cell cycle progression and have implications for understanding the transition from a quiescent to a proliferative state.

PubMed ID: 21394081
PMC ID: PMC3077088
Article link: Nat Cell Biol
Grant support: [+]
Genes referenced: actl6a cdk1 crhbp dynll1 gabrg2 ift20 ift88 myc nde1 prkci rbbp6
Morpholinos: gabrg2 MO1


Article Images: [+] show captions
References [+] :
Baker, IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia. 2003, Pubmed


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