Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Aquaculture
2011 Oct 01;3193-4:342-346. doi: 10.1016/j.aquaculture.2011.07.021.
Show Gene links
Show Anatomy links
Tol2-mediated transgenesis in tilapia (Oreochromis niloticus).
Fujimura K
,
Kocher TD
.
???displayArticle.abstract???
The Nile tilapia (Oreochromis niloticus) is an important species in aquaculture and an excellent model system for laboratory studies. Functional genetic analysis using this species has been difficult because existing methods for producing transgenics are inefficient. Here we show that the Tol2 transposon system can be used to create transgenic tilapia with high efficiency. We constructed a line that is transgenic for GFP under control of a Xenopus elongation factor 1α (EF1α) promoter. The germline transmission rate of the Tol2 construct to the first generation was about 30%, which is much higher than conventional methods. GFP expression was strong and ubiquitous in the embryos. Application of the Tol2 system for constructing transgenics in tilapia and related species will promote research in many areas, but will be especially useful for studies of evolutionary developmental biology in the cichlid fishes of East Africa.
Albertson,
Integration and evolution of the cichlid mandible: the molecular basis of alternate feeding strategies.
2005, Pubmed
Albertson,
Integration and evolution of the cichlid mandible: the molecular basis of alternate feeding strategies.
2005,
Pubmed
Albertson,
Genetic and developmental basis of cichlid trophic diversity.
2006,
Pubmed
Fisher,
Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish.
2006,
Pubmed
Fujimura,
Bone development in the jaw of Nile tilapia Oreochromis niloticus (Pisces: Cichlidae).
2008,
Pubmed
Fujimura,
Development of the embryo, larva and early juvenile of Nile tilapia Oreochromis niloticus (Pisces: Cichlidae). Developmental staging system.
2007,
Pubmed
Fujimura,
Shaping of the lower jaw bone during growth of Nile tilapia Oreochromis niloticus and a Lake Victoria cichlid Haplochromis chilotes: a geometric morphometric approach.
2008,
Pubmed
Hamlet,
Tol2 transposon-mediated transgenesis in Xenopus tropicalis.
2006,
Pubmed
,
Xenbase
Hofmann,
The eyes have it: regulatory and structural changes both underlie cichlid visual pigment diversity.
2009,
Pubmed
Ivics,
Transposon-mediated genome manipulation in vertebrates.
2009,
Pubmed
Katagiri,
A BAC-based physical map of the Nile tilapia genome.
2005,
Pubmed
Kawakami,
Excision of the tol2 transposable element of the medaka fish, Oryzias latipes, in zebrafish, Danio rerio.
1998,
Pubmed
Kawakami,
Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage.
2000,
Pubmed
Kawakami,
Transgenesis and gene trap methods in zebrafish by using the Tol2 transposable element.
2004,
Pubmed
Kawakami,
Identification of the Tol2 transposase of the medaka fish Oryzias latipes that catalyzes excision of a nonautonomous Tol2 element in zebrafish Danio rerio.
1999,
Pubmed
Kawakami,
A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish.
2004,
Pubmed
Kawakami,
Tol2: a versatile gene transfer vector in vertebrates.
2007,
Pubmed
,
Xenbase
Kijimoto,
cimp1, a novel astacin family metalloproteinase gene from East African cichlids, is differentially expressed between species during growth.
2005,
Pubmed
Kinoshita,
Activity of the medaka translation elongation factor 1alpha-A promoter examined using the GFP gene as a reporter.
2000,
Pubmed
Kocher,
Adaptive evolution and explosive speciation: the cichlid fish model.
2004,
Pubmed
Kocher,
A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus).
1998,
Pubmed
Koga,
Transposable element in fish.
1996,
Pubmed
Lee,
A second-generation genetic linkage map of tilapia (Oreochromis spp.).
2005,
Pubmed
Maclean,
Transgenic tilapia and the tilapia genome.
2002,
Pubmed
McCormick,
Calcium uptake in the skin of a freshwater teleost.
1992,
Pubmed
Negrutskii,
Eukaryotic translation elongation factor 1 alpha: structure, expression, functions, and possible role in aminoacyl-tRNA channeling.
1998,
Pubmed
Oliveira,
Molecular cytogenetic analysis of heterochromatin in the chromosomes of tilapia, Oreochromis niloticus (Teleostei: Cichlidae).
1998,
Pubmed
Parhar,
Thyroid hormone and estrogen regulate brain region-specific messenger ribonucleic acids encoding three gonadotropin-releasing hormone genes in sexually immature male fish, Oreochromis niloticus.
2000,
Pubmed
Rahman,
Copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (Oreochromis niloticus).
2000,
Pubmed
Rahman,
Expression of a novel piscine growth hormone gene results in growth enhancement in transgenic tilapia (Oreochromis niloticus).
1998,
Pubmed
Roberts,
Sexual conflict resolved by invasion of a novel sex determiner in Lake Malawi cichlid fishes.
2009,
Pubmed
Santini,
Organization and base composition of tilapia Hox genes: implications for the evolution of Hox clusters in fish.
2005,
Pubmed
Udvadia,
Windows into development: historic, current, and future perspectives on transgenic zebrafish.
2003,
Pubmed
Urasaki,
Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition.
2006,
Pubmed
Urasaki,
Transposition of the vertebrate Tol2 transposable element in Drosophila melanogaster.
2008,
Pubmed
Wilson,
Position effects on eukaryotic gene expression.
1990,
Pubmed