Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nat Commun
2011 Jan 01;2:399. doi: 10.1038/ncomms1409.
Show Gene links
Show Anatomy links
Outlines of the pore in open and closed conformations describe the gating mechanism of ASIC1.
Li T
,
Yang Y
,
Canessa CM
.
???displayArticle.abstract???
The proton-activated sodium channel ASIC1 belongs to the ENaC/Degenerins family of ion channels. Little is known about gating of the pore in any member of this class. Here we outline the shape of the ion pathway of ASIC1 in the open and closed conformations by measuring apparent rates of cysteine modification by thiol-specific reagents in the two transmembrane helices that form the pore (TM1 and TM2). Closed channels have a narrowing in the external end of the pore, whereas open channels have a narrowing midway, the length of TM2 that serves as selectivity filter. Thus, gating of the pore entails straightening the tilt of TM2 without significant rotation. The findings imply that the external narrowing serves as opening, closing and desensitization gate, and that the selectivity filter of ASIC1 is a transient structure that assembles in the open state and is pulled apart in the closed state.
Akabas,
Acetylcholine receptor channel structure probed in cysteine-substitution mutants.
1992,
Pubmed
,
Xenbase
Benos,
Cationic selectivity and competition at the sodium entry site in frog skin.
1980,
Pubmed
Evans,
Ionic permeability of, and divalent cation effects on, two ATP-gated cation channels (P2X receptors) expressed in mammalian cells.
1996,
Pubmed
Gonzales,
Pore architecture and ion sites in acid-sensing ion channels and P2X receptors.
2009,
Pubmed
Holmgren,
On the use of thiol-modifying agents to determine channel topology.
1996,
Pubmed
,
Xenbase
Jasti,
Structure of acid-sensing ion channel 1 at 1.9 A resolution and low pH.
2007,
Pubmed
Karlin,
Substituted-cysteine accessibility method.
1998,
Pubmed
Kawate,
Crystal structure of the ATP-gated P2X(4) ion channel in the closed state.
2009,
Pubmed
Kellenberger,
Permeability properties of ENaC selectivity filter mutants.
2001,
Pubmed
,
Xenbase
Kellenberger,
On the molecular basis of ion permeation in the epithelial Na+ channel.
1999,
Pubmed
,
Xenbase
Li,
Asp433 in the closing gate of ASIC1 determines stability of the open state without changing properties of the selectivity filter or Ca2+ block.
2011,
Pubmed
,
Xenbase
Li,
Pore-opening mechanism in trimeric P2X receptor channels.
2010,
Pubmed
Li,
Two residues in the extracellular domain convert a nonfunctional ASIC1 into a proton-activated channel.
2010,
Pubmed
,
Xenbase
Li,
Gating the pore of P2X receptor channels.
2008,
Pubmed
Palmer,
Epithelial Na channels: the nature of the conducting pore.
1990,
Pubmed
Roux,
Ion selectivity in channels and transporters.
2011,
Pubmed
Sluka,
Acid-sensing ion channels: A new target for pain and CNS diseases.
2009,
Pubmed
Wemmie,
The acid-activated ion channel ASIC contributes to synaptic plasticity, learning, and memory.
2002,
Pubmed
Zhang,
Gating of acid-sensitive ion channel-1: release of Ca2+ block vs. allosteric mechanism.
2006,
Pubmed
,
Xenbase
