Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract??? Prominin-1 expresses in rod and cone photoreceptors. Mutations in the prominin-1 gene cause retinal degeneration in humans. In this study, the authors investigated the expression and subcellular localization of xlProminin-1 protein, the Xenopus laevis ortholog of prominin-1, in rod and cone photoreceptors of this frog. Antibodies specific for xlProminin-1 were generated. Immunoblotting was used to study the expression and posttranslational processing of xlProminin-1 protein. Immunocytochemical light and electron microscopy and transgenesis were used to study the subcellular distribution of xlProminin-1. xlProminin-1 is expressed and is subject to posttranslational proteolytic processing in the retina, brain, and kidney. xlProminin-1 is differently expressed and localized in outer segments of rod and cone photoreceptors of X. laevis. Antibodies specific for the N or C termini of xlProminin-1 labeled the open rims of lamellae of cone outer segments (COS) and the open lamellae at the base of rod outer segments (ROS). By contrast, anti-peripherin-2/rds antibody, Xper5A11, labeled the closed rims of cone lamellae adjacent to the ciliary axoneme and the rims of the closed ROS disks. The extent of labeling of the basal ROS by anti-xlProminin-1 antibodies varied with the light cycle in this frog. The entire ROS was also faintly labeled by both antibodies, a result that contrasts with the current notion that prominin-1 localizes only to the basal ROS. These findings suggest that xlProminin-1 may serve as an anti-fusogenic factor in the regulation of disk morphogenesis and may help to maintain the open lamellar structure of basal ROS and COS disks in X. laevis photoreceptors.
Anderson,
Mammalian cones: disc shedding, phagocytosis, and renewal.
1978, Pubmed
Anderson,
Mammalian cones: disc shedding, phagocytosis, and renewal.
1978,
Pubmed
Arikawa,
Localization of peripherin/rds in the disk membranes of cone and rod photoreceptors: relationship to disk membrane morphogenesis and retinal degeneration.
1992,
Pubmed
Besharse,
Turnover of rod photoreceptor outer segments. II. Membrane addition and loss in relationship to light.
1977,
Pubmed
,
Xenbase
Boesze-Battaglia,
Fusion between retinal rod outer segment membranes and model membranes: functional assays and role for peripherin/rds.
2000,
Pubmed
Boesze-Battaglia,
A soluble peripherin/Rds C-terminal polypeptide promotes membrane fusion and changes conformation upon membrane association.
2003,
Pubmed
Boesze-Battaglia,
Fusion between retinal rod outer segment membranes and model membranes: a role for photoreceptor peripherin/rds.
1998,
Pubmed
Boesze-Battaglia,
ROM-1 potentiates photoreceptor specific membrane fusion processes.
2007,
Pubmed
Bowmaker,
Visual pigments and colour vision in man and monkeys.
1981,
Pubmed
Braekevelt,
Photoreceptor fine structure in Oreochromis niloticus L. (Cichlidae; Teleostei) in light- and dark-adaptation.
1998,
Pubmed
COHEN,
NEW DETAILS OF THE ULTRASTRUCTURE OF THE OUTER SEGMENTS AND CILIARY CONNECTIVES OF THE RODS OF HUMAN AND MACAQUE RETINAS.
1965,
Pubmed
Chuang,
SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods.
2007,
Pubmed
Cohen,
New evidence supporting the linkage to extracellular space of outer segment saccules of frog cones but not rods.
1968,
Pubmed
Corbeil,
Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein.
2001,
Pubmed
Damek-Poprawa,
A novel tetraspanin fusion protein, peripherin-2, requires a region upstream of the fusion domain for activity.
2005,
Pubmed
Deretic,
Phosphoinositides, ezrin/moesin, and rac1 regulate fusion of rhodopsin transport carriers in retinal photoreceptors.
2004,
Pubmed
Deretic,
rab8 in retinal photoreceptors may participate in rhodopsin transport and in rod outer segment disk morphogenesis.
1995,
Pubmed
Eckmiller,
Cone outer segment morphogenesis: taper change and distal invaginations.
1987,
Pubmed
,
Xenbase
Edrington,
Peripherin-2: an intracellular analogy to viral fusion proteins.
2007,
Pubmed
Fiedler,
An engineered IN-1 F(ab) fragment with improved affinity for the Nogo-A axonal growth inhibitor permits immunochemical detection and shows enhanced neutralizing activity.
2002,
Pubmed
Goldberg,
Cysteine residues of photoreceptor peripherin/rds: role in subunit assembly and autosomal dominant retinitis pigmentosa.
1998,
Pubmed
Han,
Identification of three prominin homologs and characterization of their messenger RNA expression in Xenopus laevis tissues.
2011,
Pubmed
,
Xenbase
Kedzierski,
Three homologs of rds/peripherin in Xenopus laevis photoreceptors that exhibit covalent and non-covalent interactions.
1996,
Pubmed
,
Xenbase
Kedzierski,
Analysis of the rds/peripherin.rom1 complex in transgenic photoreceptors that express a chimeric protein.
1999,
Pubmed
Kozak,
An analysis of vertebrate mRNA sequences: intimations of translational control.
1991,
Pubmed
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lamb,
Evolution of the vertebrate eye: opsins, photoreceptors, retina and eye cup.
2007,
Pubmed
Laties,
Cones of living amphibian eye: selective staining.
1970,
Pubmed
Laties,
Procion yellow: a marker dye for outer segment disc patency and for rod renewal.
1976,
Pubmed
Loewen,
The role of subunit assembly in peripherin-2 targeting to rod photoreceptor disk membranes and retinitis pigmentosa.
2003,
Pubmed
,
Xenbase
Matsumoto,
Light and temperature modulated staining of the rod outer segment distal tips with Lucifer yellow.
1985,
Pubmed
,
Xenbase
Maw,
A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration.
2000,
Pubmed
Miraglia,
A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning.
1997,
Pubmed
Moritz,
Fluorescent photoreceptors of transgenic Xenopus laevis imaged in vivo by two microscopy techniques.
1999,
Pubmed
,
Xenbase
Moritz,
Mutant rab8 Impairs docking and fusion of rhodopsin-bearing post-Golgi membranes and causes cell death of transgenic Xenopus rods.
2001,
Pubmed
,
Xenbase
Moritz,
A functional rhodopsin-green fluorescent protein fusion protein localizes correctly in transgenic Xenopus laevis retinal rods and is expressed in a time-dependent pattern.
2001,
Pubmed
,
Xenbase
Nathans,
Molecular genetics of human color vision: the genes encoding blue, green, and red pigments.
1986,
Pubmed
Papermaster,
Membrane biosynthesis in the frog retina: opsin transport in the photoreceptor cell.
1975,
Pubmed
Papermaster,
Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells. An electron microscope analysis of cross-linked albumin-embedded retinas.
1978,
Pubmed
Papermaster,
Preparation of retinal rod outer segments.
1982,
Pubmed
Papermaster,
Vesicular transport of newly synthesized opsin from the Golgi apparatus toward the rod outer segment. Ultrastructural immunocytochemical and autoradiographic evidence in Xenopus retinas.
1985,
Pubmed
,
Xenbase
Papermaster,
Biosynthesis and vectorial transport of opsin on vesicles in retinal rod photoreceptors.
1986,
Pubmed
,
Xenbase
Permanyer,
Autosomal recessive retinitis pigmentosa with early macular affectation caused by premature truncation in PROM1.
2010,
Pubmed
Peters,
Fine structure of a periciliary ridge complex of frog retinal rod cells revealed by ultrahigh resolution scanning electron microscopy.
1983,
Pubmed
Peterson,
Arrestin migrates in photoreceptors in response to light: a study of arrestin localization using an arrestin-GFP fusion protein in transgenic frogs.
2003,
Pubmed
,
Xenbase
Poirier,
A ts T mutant of Schmidt Ruppin strain of Rous sarcoma virus restricted at 39.5 degrees C for the morphological transformation and the tumorigenicity of chicken embryo fibroblasts.
1982,
Pubmed
Roepman,
Protein networks and complexes in photoreceptor cilia.
2007,
Pubmed
Steinberg,
Disc morphogenesis in vertebrate photoreceptors.
1980,
Pubmed
Wald,
The molecular basis of visual excitation.
1968,
Pubmed
Weigmann,
Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells.
1997,
Pubmed
Yang,
Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice.
2008,
Pubmed
Yau,
Phototransduction mechanism in retinal rods and cones. The Friedenwald Lecture.
1994,
Pubmed
Yin,
AC133, a novel marker for human hematopoietic stem and progenitor cells.
1997,
Pubmed
Young,
The renewal of photoreceptor cell outer segments.
1967,
Pubmed
Young,
Passage of newly formed protein through the connecting cilium of retina rods in the frog.
1968,
Pubmed
Young,
The renewal of protein in retinal rods and cones.
1968,
Pubmed
Young,
Participation of the retinal pigment epithelium in the rod outer segment renewal process.
1969,
Pubmed
Young,
Autoradiographic studies on the metabolism of the retinal pigment epithelium.
1970,
Pubmed
Young,
Visual cells and the concept of renewal.
1976,
Pubmed
Zacchigna,
Loss of the cholesterol-binding protein prominin-1/CD133 causes disk dysmorphogenesis and photoreceptor degeneration.
2009,
Pubmed
Zelhof,
Transforming the architecture of compound eyes.
2006,
Pubmed
