CSH Protoc. January 1, 2007; 2007
Ectodermal (Animal Cap) Layer Separations in Xenopus laevis.
AbstractINTRODUCTIONIn Xenopus laevis, the blastula animal cap comprises two morphologically distinct cell layers, an outer monolayer termed the epithelial layer, which consists of tightly adherent pigmented cells, and an inner layer several cells thick termed the sensorial layer, which consists of loosely adherent cells. It is possible to isolate cell layers and to test their developmental potential and response to induction. This protocol describes how to separate the cell layers most easily, that is, by dissecting an intact Xenopus embryo.
Pubmed Id: 21357094Article link: CSH Protoc.