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XB-ART-44682
Dev Biol. March 1, 2012; 363 (1): 155-65.

RFX2 is broadly required for ciliogenesis during vertebrate development.

Chung MI , Peyrot SM , LeBoeuf S , Park TJ , McGary KL , Marcotte EM , Wallingford JB .


Abstract
In Caenorhabditis elegans, the RFX (Daf19) transcription factor is a major regulator of ciliogenesis, controlling the expression of the many essential genes required for making cilia. In vertebrates, however, seven RFX genes have been identified. Bioinformatic analysis suggests that Rfx2 is among the closest homologues of Daf19. We therefore hypothesize that Rfx2 broadly controls ciliogenesis during vertebrate development. Indeed, here we show that Rfx2 in Xenopus is expressed preferentially in ciliated tissues, including neural tube, gastrocoel roof plate, epidermal multi-ciliated cells, otic vesicles, and kidneys. Knockdown of Rfx2 results in cilia-defective embryonic phenotypes and fewer or truncated cilia are observed in Rfx2 morphants. These results indicate that Rfx2 is broadly required for ciliogenesis in vertebrates. Furthermore, we show that Rfx2 is essential for expression of several ciliogenic genes, including TTC25, which we show here is required for ciliogenesis, HH signaling, and left-right patterning.

PubMed ID: 22227339
PMC ID: PMC3640985
Article link: Dev Biol.
Grant support: R01 GM067779-08 NIGMS NIH HHS , R01 GM088624 NIGMS NIH HHSHoward Hughes Medical Institute , R01 GM067779 NIGMS NIH HHS , R01 GM074104 NIGMS NIH HHS , R01 GM067779 NIGMS NIH HHS , R01 GM074104 NIGMS NIH HHS , R01 GM088624 NIGMS NIH HHSHoward Hughes Medical Institute

Genes referenced: arl13b cdh1 foxj1 lefty nkx2-2 pitx2 rfx1 rfx2 rfx3 rfx4 rfx5 shh tac1 ttc25 tuba4a vax1 tuba8

Antibodies referenced: Arl13b Ab1 Tuba4b Ab3
Morpholinos referenced: rfx2 MO1 ttc25 MO1

References:
Aftab, 2008, Pubmed[+]


Article Images: [+] show captions

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