Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Channels (Austin)
2011 Jan 01;54:345-56. doi: 10.4161/chan.5.4.17082.
Show Gene links
Show Anatomy links
Fourier transform coupled tryptophan scanning mutagenesis identifies a bending point on the lipid-exposed δM3 transmembrane domain of the Torpedo californica nicotinic acetylcholine receptor.
Caballero-Rivera D
,
Cruz-Nieves OA
,
Oyola-Cintrón J
,
Torres-Núñez DA
,
Otero-Cruz JD
,
Lasalde-Dominicci JA
.
???displayArticle.abstract???
The nicotinic acetylcholine receptor (nAChR) is a member of a family of ligand-gated ion channels that mediate diverse physiological functions, including fast synaptic transmission along the peripheral and central nervous systems. Several studies have made significant advances toward determining the structure and dynamics of the lipid-exposed domains of the nAChR. However, a high-resolution atomic structure of the nAChR still remains elusive. In this study, we extended the Fourier transform coupled tryptophan scanning mutagenesis (FT-TrpScanM) approach to gain insight into the secondary structure of the δM3 transmembrane domain of the Torpedo californica nAChR, to monitor conformational changes experienced by this domain during channel gating, and to identify which lipid-exposed positions are linked to the regulation of ion channel kinetics. The perturbations produced by periodic tryptophan substitutions along the δM3 transmembrane domain were characterized by two-electrode voltage clamp and (125)I-labeled α-bungarotoxin binding assays. The periodicity profiles and Fourier transform spectra of this domain revealed similar helical structures for the closed- and open-channel states. However, changes in the oscillation patterns observed between positions Val-299 and Val-304 during transition between the closed- and open-channel states can be explained by the structural effects caused by the presence of a bending point introduced by a Thr-Gly motif at positions 300-301. The changes in periodicity and localization of residues between the closed-and open-channel states could indicate a structural transition between helix types in this segment of the domain. Overall, the data further demonstrate a functional link between the lipid-exposed transmembrane domain and the nAChR gating machinery.
Akabas,
Identification of acetylcholine receptor channel-lining residues in the M1 segment of the alpha-subunit.
1995, Pubmed,
Xenbase
Akabas,
Identification of acetylcholine receptor channel-lining residues in the M1 segment of the alpha-subunit.
1995,
Pubmed
,
Xenbase
Baenziger,
Fourier transform infrared and hydrogen/deuterium exchange reveal an exchange-resistant core of alpha-helical peptide hydrogens in the nicotinic acetylcholine receptor.
1995,
Pubmed
Barrantes,
Structural basis for lipid modulation of nicotinic acetylcholine receptor function.
2004,
Pubmed
Blanton,
Mapping the lipid-exposed regions in the Torpedo californica nicotinic acetylcholine receptor.
1992,
Pubmed
Blanton,
Identifying the lipid-protein interface of the Torpedo nicotinic acetylcholine receptor: secondary structure implications.
1994,
Pubmed
Blanton,
Probing the structure of the nicotinic acetylcholine receptor ion channel with the uncharged photoactivable compound -3H-diazofluorene.
1998,
Pubmed
Brannigan,
Embedded cholesterol in the nicotinic acetylcholine receptor.
2008,
Pubmed
Brejc,
Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors.
2001,
Pubmed
Caballero-Herrera,
Effect of urea on peptide conformation in water: molecular dynamics and experimental characterization.
2005,
Pubmed
Campos-Caro,
Role of the putative transmembrane segment M3 in gating of neuronal nicotinic receptors.
1997,
Pubmed
,
Xenbase
Chang,
Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel.
1998,
Pubmed
Choe,
Three distinct structural environments of a transmembrane domain in the inwardly rectifying potassium channel ROMK1 defined by perturbation.
1995,
Pubmed
,
Xenbase
Choi,
Structural studies of metarhodopsin II, the activated form of the G-protein coupled receptor, rhodopsin.
2002,
Pubmed
Chothia,
Structural invariants in protein folding.
1975,
Pubmed
Collins,
Scanning mutagenesis of the putative transmembrane segments of Kir2.1, an inward rectifier potassium channel.
1997,
Pubmed
,
Xenbase
Cruz-Martín,
Tryptophan substitutions at lipid-exposed positions of the gamma M3 transmembrane domain increase the macroscopic ionic current response of the Torpedo californica nicotinic acetylcholine receptor.
2001,
Pubmed
,
Xenbase
Cukras,
Structural and functional determinants of conserved lipid interaction domains of inward rectifying Kir6.2 channels.
2002,
Pubmed
Cymes,
Pore-opening mechanism of the nicotinic acetylcholine receptor evinced by proton transfer.
2008,
Pubmed
De Rosa,
Nicotinic receptor M3 transmembrane domain: position 8' contributes to channel gating.
2002,
Pubmed
Dellisanti,
Crystal structure of the extracellular domain of nAChR alpha1 bound to alpha-bungarotoxin at 1.94 A resolution.
2007,
Pubmed
Duneau,
Detailed description of an alpha helix-->pi bulge transition detected by molecular dynamics simulations of the p185c-erbB2 V659G transmembrane domain.
1996,
Pubmed
Díaz-De León,
Tryptophan scanning of the acetylcholine receptor's betaM4 transmembrane domain: decoding allosteric linkage at the lipid-protein interface with ion-channel gating.
2008,
Pubmed
Grosman,
Mapping the conformational wave of acetylcholine receptor channel gating.
2000,
Pubmed
Grosman,
The extracellular linker of muscle acetylcholine receptor channels is a gating control element.
2000,
Pubmed
Guzmán,
Tryptophan scanning mutagenesis in the alphaM3 transmembrane domain of the Torpedo californica acetylcholine receptor: functional and structural implications.
2003,
Pubmed
,
Xenbase
Honse,
Sites in the fourth membrane-associated domain regulate alcohol sensitivity of the NMDA receptor.
2004,
Pubmed
Irizarry,
Opening the KcsA K+ channel: tryptophan scanning and complementation analysis lead to mutants with altered gating.
2002,
Pubmed
Jenkins,
Tryptophan scanning mutagenesis in TM4 of the GABA(A) receptor alpha1 subunit: implications for modulation by inhaled anesthetics and ion channel structure.
2002,
Pubmed
Jiang,
The open pore conformation of potassium channels.
2002,
Pubmed
Kalamida,
Muscle and neuronal nicotinic acetylcholine receptors. Structure, function and pathogenicity.
2007,
Pubmed
Karlin,
Emerging structure of the nicotinic acetylcholine receptors.
2002,
Pubmed
Lasalde,
Tryptophan substitutions at the lipid-exposed transmembrane segment M4 of Torpedo californica acetylcholine receptor govern channel gating.
1996,
Pubmed
,
Xenbase
Lee,
Mutations in the M4 domain of Torpedo californica acetylcholine receptor dramatically alter ion channel function.
1994,
Pubmed
,
Xenbase
Li,
Functional role of the cysteine 451 thiol group in the M4 helix of the gamma subunit of Torpedo californica acetylcholine receptor.
1990,
Pubmed
,
Xenbase
Li,
Site-specific mutations of nicotinic acetylcholine receptor at the lipid-protein interface dramatically alter ion channel gating.
1992,
Pubmed
,
Xenbase
Li-Smerin,
alpha-helical structural elements within the voltage-sensing domains of a K(+) channel.
2000,
Pubmed
,
Xenbase
Li-Smerin,
A localized interaction surface for voltage-sensing domains on the pore domain of a K+ channel.
2000,
Pubmed
,
Xenbase
Li-Smerin,
Helical structure of the COOH terminus of S3 and its contribution to the gating modifier toxin receptor in voltage-gated ion channels.
2001,
Pubmed
Lugovskoy,
Spatial structure of the M3 transmembrane segment of the nicotinic acetylcholine receptor alpha subunit.
1998,
Pubmed
Miyazawa,
Structure and gating mechanism of the acetylcholine receptor pore.
2003,
Pubmed
Méthot,
Secondary structure of the exchange-resistant core from the nicotinic acetylcholine receptor probed directly by infrared spectroscopy and hydrogen/deuterium exchange.
1998,
Pubmed
Navedo,
Tryptophan substitutions reveal the role of nicotinic acetylcholine receptor alpha-TM3 domain in channel gating: differences between Torpedo and muscle-type AChR.
2004,
Pubmed
,
Xenbase
Ortiz-Acevedo,
Tryptophan scanning mutagenesis of the gammaM4 transmembrane domain of the acetylcholine receptor from Torpedo californica.
2004,
Pubmed
,
Xenbase
Ortiz-Miranda,
Mutations in the M4 domain of the Torpedo californica nicotinic acetylcholine receptor alter channel opening and closing.
1997,
Pubmed
,
Xenbase
Otero-Cruz,
Tryptophan-scanning mutagenesis in the alphaM3 transmembrane domain of the muscle-type acetylcholine receptor. A spring model revealed.
2007,
Pubmed
,
Xenbase
Otero-Cruz,
Fourier transform coupled to tryptophan-scanning mutagenesis: lessons from its application to the prediction of secondary structure in the acetylcholine receptor lipid-exposed transmembrane domains.
2008,
Pubmed
,
Xenbase
Powl,
Identification of the hydrophobic thickness of a membrane protein using fluorescence spectroscopy: studies with the mechanosensitive channel MscL.
2005,
Pubmed
Ren,
Visualization of a water-selective pore by electron crystallography in vitreous ice.
2001,
Pubmed
Riek,
Wide turn diversity in protein transmembrane helices implications for G-protein-coupled receptor and other polytopic membrane protein structure and function.
2008,
Pubmed
Santiago,
Tryptophan scanning mutagenesis in the TM3 domain of the Torpedo californica acetylcholine receptor beta subunit reveals an alpha-helical structure.
2004,
Pubmed
,
Xenbase
Silberberg,
Secondary structure and gating rearrangements of transmembrane segments in rat P2X4 receptor channels.
2005,
Pubmed
,
Xenbase
Tamamizu,
Alteration in ion channel function of mouse nicotinic acetylcholine receptor by mutations in the M4 transmembrane domain.
1999,
Pubmed
,
Xenbase
Tamamizu,
Functional effects of periodic tryptophan substitutions in the alpha M4 transmembrane domain of the Torpedo californica nicotinic acetylcholine receptor.
2000,
Pubmed
,
Xenbase
Tieleman,
Proline-induced hinges in transmembrane helices: possible roles in ion channel gating.
2001,
Pubmed
Toyoshima,
Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution.
2000,
Pubmed
Unwin,
Refined structure of the nicotinic acetylcholine receptor at 4A resolution.
2005,
Pubmed
Vos,
Structure of membrane-embedded M13 major coat protein is insensitive to hydrophobic stress.
2007,
Pubmed
Wang,
Tryptophan scanning of D1S6 and D4S6 C-termini in voltage-gated sodium channels.
2003,
Pubmed
Wang,
Acetylcholine receptor M3 domain: stereochemical and volume contributions to channel gating.
1999,
Pubmed
Zhu,
Gating transition of pentameric ligand-gated ion channels.
2009,
Pubmed
