XB-ART-44732
Gen Comp Endocrinol
July 1, 2012;
177
(3):
315-21.
The role of brain-derived neurotrophic factor in the regulation of cell growth and gene expression in melanotrope cells of Xenopus laevis.
Abstract
Brain-derived neurotrophic factor (
BDNF) is, despite its name, also found outside the
central nervous system (
CNS), but the functional significance of this observation is largely unknown. This review concerns the expression of
BDNF in the
pituitary gland. While the presence of the neurotrophin in the mammalian
pituitary gland is well documented its functional significance remains obscure. Studies on the
pars intermedia of the
pituitary of the amphibian Xenopus laevis have shown that
BDNF is produced by the neuroendocrine
melanotrope cells, its expression is physiologically regulated, and the
melanotrope cells themselves express receptors for the neurotrophin. The neurotrophin has been shown to act as an autocrine factor on the
melanotrope to promote cell growth and regulate gene expression. In doing so
BDNF supports the physiological function of the cell to produce and release α-
melanophore-stimulating hormone for the purpose of adjusting the animal''s
skin color to that of its background.
PubMed ID:
22248443
Article link:
Gen Comp Endocrinol
Genes referenced:
adcyap1
bdnf
creb1
crh
fos
kcnip3
mapk1
npy
nr4a1
ntrk2
pomc
vipr1
Article Images:
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Fig. 1. Schematic overview of the regulation of the melanotrope cell of Xenopus laevis. The secretion of α-MSH is regulated by multiple neurotransmitters, some of which are indicated in the figure, together with their receptors that activate (cyan) or inhibit (red) adenylyl cyclase. Cyclic-AMP binds to the regulatory subunit (R) of PKA leading to the dissociation of the catalytic subunit (C) which then activates components of Ca2+ signaling machinery on the plasma membrane, resulting in opening of voltage-operated Ca2+ channels (VOCC) and influx of Ca2+. This Ca2+ signal stimulates exocytosis and mobilizes intracellular Ca2+ stores to initiate a self-propagating wave of Ca2+ that ultimately enters the nucleus. In the nucleus Ca2+ and the catalytic subunit of PKA are responsible for activating transcription factors (e.g. CREB, USF1/2, CaRF1) that act on responsive elements (grey boxes) within the promoters of various genes. Among the first genes to be activated are the immediate early genes c-Fos and Nur77, along with BDNF, POMC and proBDNF mRNA are translated at the rough endoplasmic reticulum (RER) and co-packaged into secretory granules. Mature BDNF acts as an autocrine factor, with TrkB signaling activating ERK that subsequently phosphorylates DCLK-short to translocate into the nucleus. In the nucleus DCLK-short stimulates, through unknown pathways, the expression of POMC; it is likely also involved in the regulation of other genes, such as those governing cell growth. ERK may act via Nur77 on POMC expression, possibly in a compartment regulated by PKA associated with AKAP. Abbreviations: AKAP, A kinase-associated protein; AP1, activator protein 1 promoter site; BDNF, brain-derived neurotrophic factor; β, β-adrenergic receptor; β/γ, β/γ subunit of G proteins; CaRE, calcium-responsive element; CaRF, calcium-responsive transcription factor; CRE, cyclic-AMP-responsive element; CRH, corticotropin-releasing hormone; D2, dopamine D2 receptor; DCLK, doublecortin-like kinase; DRE, down-stream responsive element; DREAM, DRE antagonist modulator; ERK, extracellular-signal-regulated kinase; Gb, GABAb receptor; NPY, neuropeptide Y; PACAP, pituitary adenylate cyclase-activating polypeptide; POMC, proopiomelanocortin; PLCγ, phospholipase Cγ; PKA, protein kinase A; R1, CRH receptor 1; USF, upstream stimulatory factor; V1, VPAC1 receptor; VOCC, voltage-operated Ca2+ channel; Y1, NPY Y1 receptor. (Adapted from Kidane [33] and Kuribara [43].
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