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JAM-A protects from thrombosis by suppressing integrin αIIbβ3-dependent outside-in signaling in platelets.
Naik MU
,
Stalker TJ
,
Brass LF
,
Naik UP
.
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Mounting evidence suggests that agonist-initiated signaling in platelets is closely regulated to avoid excessive responses to injury. A variety of physiologic agonists induce a cascade of signaling events termed as inside-out signaling that culminate in exposure of high-affinity binding sites on integrin α(IIb)β(3). Once platelet activation has occurred, integrin α(IIb)β(3) stabilizes thrombus formation by providing agonist-independent "outside-in" signals mediated in part by contractile signaling. Junctional adhesion molecule A (JAM-A), a member of the cortical thymocyte marker of the Xenopus (CTX) family, was initially identified as a receptor for a platelet stimulatory mAb. Here we show that JAM-A in resting platelets functions as an endogenous inhibitor of platelet function. Genetic ablation of Jam-A in mice enhances thrombotic function of platelets in vivo. The absence of Jam-A results in increase in platelet aggregation ex vivo. This gain of function is not because of enhanced inside-out signaling because granular secretion, Thromboxane A2 (TxA2) generation, as well as fibrinogen receptor activation, are normal in the absence of Jam-A. Interestingly, integrin outside-in signaling such as platelet spreading and clot retraction is augmented in Jam-A-deficient platelets. We conclude that JAM-A normally limits platelet accumulation by inhibiting integrin outside-in signaling thus preventing premature platelet activation.
Ablooglu,
Antithrombotic effects of targeting alphaIIbbeta3 signaling in platelets.
2009, Pubmed
Ablooglu,
Antithrombotic effects of targeting alphaIIbbeta3 signaling in platelets.
2009,
Pubmed
Angelillo-Scherrer,
Deficiency or inhibition of Gas6 causes platelet dysfunction and protects mice against thrombosis.
2001,
Pubmed
Aurrand-Lions,
Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members.
2001,
Pubmed
,
Xenbase
Babinska,
Two regions of the human platelet F11-receptor (F11R) are critical for platelet aggregation, potentiation and adhesion.
2002,
Pubmed
Babinska,
F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells: role in inflammatory thrombosis.
2002,
Pubmed
Bergmeier,
Flow cytometric detection of activated mouse integrin alphaIIbbeta3 with a novel monoclonal antibody.
2002,
Pubmed
Boylan,
Identification of FcgammaRIIa as the ITAM-bearing receptor mediating alphaIIbbeta3 outside-in integrin signaling in human platelets.
2008,
Pubmed
Broos,
Platelets at work in primary hemostasis.
2011,
Pubmed
Chen,
Mer receptor tyrosine kinase signaling participates in platelet function.
2004,
Pubmed
Cooke,
Fibroblast growth factor-2 failed to induce angiogenesis in junctional adhesion molecule-A-deficient mice.
2006,
Pubmed
Falati,
Platelet PECAM-1 inhibits thrombus formation in vivo.
2006,
Pubmed
Falati,
Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse.
2002,
Pubmed
Flevaris,
A molecular switch that controls cell spreading and retraction.
2007,
Pubmed
Gibbins,
The negative regulation of platelet function: extending the role of the ITIM.
2002,
Pubmed
Gibbins,
JAMming the signals.
2012,
Pubmed
Gong,
G protein subunit Galpha13 binds to integrin alphaIIbbeta3 and mediates integrin "outside-in" signaling.
2010,
Pubmed
Goodman,
Sheep, pig, and human platelet-material interactions with model cardiovascular biomaterials.
1999,
Pubmed
Henshall,
Src homology 2 domain-containing protein-tyrosine phosphatases, SHP-1 and SHP-2, are required for platelet endothelial cell adhesion molecule-1/CD31-mediated inhibitory signaling.
2001,
Pubmed
Hirata Ki,
Cloning of an immunoglobulin family adhesion molecule selectively expressed by endothelial cells.
2001,
Pubmed
Huber,
The carboxyl-terminal region of biliary glycoprotein controls its tyrosine phosphorylation and association with protein-tyrosine phosphatases SHP-1 and SHP-2 in epithelial cells.
1999,
Pubmed
Hughes,
Integrin affinity modulation.
1998,
Pubmed
Jackson,
The protein-tyrosine phosphatase SHP-2 binds platelet/endothelial cell adhesion molecule-1 (PECAM-1) and forms a distinct signaling complex during platelet aggregation. Evidence for a mechanistic link between PECAM-1- and integrin-mediated cellular signaling.
1997,
Pubmed
Jones,
Platelet endothelial cell adhesion molecule-1 is a negative regulator of platelet-collagen interactions.
2001,
Pubmed
Kang,
Deletion of JAM-A causes morphological defects in the corneal epithelium.
2007,
Pubmed
Kornecki,
Activation of human platelets by a stimulatory monoclonal antibody.
1990,
Pubmed
Li,
Signaling during platelet adhesion and activation.
2010,
Pubmed
Ling,
Annexin II regulates fibrin homeostasis and neoangiogenesis in vivo.
2004,
Pubmed
Mandell,
Junctional adhesion molecule 1 regulates epithelial cell morphology through effects on beta1 integrins and Rap1 activity.
2005,
Pubmed
Müller,
Homophilic adhesion and CEACAM1-S regulate dimerization of CEACAM1-L and recruitment of SHP-2 and c-Src.
2009,
Pubmed
Naik,
CIB1 deficiency results in impaired thrombosis: the potential role of CIB1 in outside-in signaling through integrin alpha IIb beta 3.
2009,
Pubmed
Naik,
Contra-regulation of calcium- and integrin-binding protein 1-induced cell migration on fibronectin by PAK1 and MAP kinase signaling.
2011,
Pubmed
Naik,
Association of CIB with GPIIb/IIIa during outside-in signaling is required for platelet spreading on fibrinogen.
2003,
Pubmed
Naik,
Signaling through JAM-1 and alphavbeta3 is required for the angiogenic action of bFGF: dissociation of the JAM-1 and alphavbeta3 complex.
2003,
Pubmed
Naik,
Junctional adhesion molecule 1 (JAM-1).
2003,
Pubmed
Naik,
Mechanisms of platelet activation by a stimulatory antibody: cross-linking of a novel platelet receptor for monoclonal antibody F11 with the Fc gamma RII receptor.
1995,
Pubmed
Obergfell,
Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton.
2002,
Pubmed
Ostermann,
JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes.
2002,
Pubmed
Ozaki,
Junctional adhesion molecule (JAM) is phosphorylated by protein kinase C upon platelet activation.
2000,
Pubmed
Parris,
JAM-A expression during embryonic development.
2005,
Pubmed
Patil,
Platelet endothelial cell adhesion molecule-1 serves as an inhibitory receptor that modulates platelet responses to collagen.
2001,
Pubmed
Rivera,
Platelet receptors and signaling in the dynamics of thrombus formation.
2009,
Pubmed
Shao,
JAM-A is present in mammalian spermatozoa where it is essential for normal motility.
2008,
Pubmed
Shattil,
Signaling through platelet integrin alpha IIb beta 3: inside-out, outside-in, and sideways.
1999,
Pubmed
Sobocka,
Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation.
2000,
Pubmed
Sobocka,
Signaling pathways of the F11 receptor (F11R; a.k.a. JAM-1, JAM-A) in human platelets: F11R dimerization, phosphorylation and complex formation with the integrin GPIIIa.
2004,
Pubmed
Stalker,
Endothelial cell specific adhesion molecule (ESAM) localizes to platelet-platelet contacts and regulates thrombus formation in vivo.
2009,
Pubmed
Su,
RGT, a synthetic peptide corresponding to the integrin beta 3 cytoplasmic C-terminal sequence, selectively inhibits outside-in signaling in human platelets by disrupting the interaction of integrin alpha IIb beta 3 with Src kinase.
2008,
Pubmed
Sun,
Platelet endothelial cell adhesion molecule-1 (PECAM-1) homophilic adhesion is mediated by immunoglobulin-like domains 1 and 2 and depends on the cytoplasmic domain and the level of surface expression.
1996,
Pubmed
Suzuki-Inoue,
Involvement of Src kinases and PLCgamma2 in clot retraction.
2007,
Pubmed
Weiss,
Protection against thrombosis in mice lacking PAR3.
2002,
Pubmed
Wong,
CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo.
2009,
Pubmed