XB-ART-44837Genesis. March 1, 2012; 50 (3): 164-75.
The hitchhiker''s guide to Xenopus genetics.
A decade after the human genome sequence, most vertebrate gene functions remain poorly understood, limiting benefits to human health from rapidly advancing genomic technologies. Systematic in vivo functional analysis is ideally suited to the experimentally accessible Xenopus embryo, which combines embryological accessibility with a broad range of transgenic, biochemical, and gain-of-function assays. The diploid X. tropicalis adds loss-of-function genetics and enhanced genomics to this repertoire. In the last decade, diverse phenotypes have been recovered from genetic screens, mutations have been cloned, and reverse genetics in the form of TILLING and targeted gene editing have been established. Simple haploid genetics and gynogenesis and the very large number of embryos produced streamline screening and mapping. Improved genomic resources and the revolution in high-throughput sequencing are transforming mutation cloning and reverse genetic approaches. The combination of loss-of-function mutant backgrounds with the diverse array of conventional Xenopus assays offers a uniquely flexible platform for analysis of gene function in vertebrate development.
PubMed ID: 22344745
PMC ID: PMC3312310
Article link: Genesis.
Grant support: R01 DC011901-01 NIDCD NIH HHS , R01 DE018825-01A1 NIDCR NIH HHS, R01 HD054354-01 NICHD NIH HHS , 1R01 HD054354-01 NICHD NIH HHS , 1R01DC011901-01 NIDCD NIH HHS , 1R01DE018824 NIDCR NIH HHS, 1R01DE018825-01A1 NIDCR NIH HHS, U117560482 Medical Research Council , R01 DE018824-02S1 NIDCR NIH HHS, R01 DE018825 NIDCR NIH HHS, R01 GM099149 NIGMS NIH HHS , MC_U117560482 Medical Research Council , P40 OD010997 NIH HHS , R01 DC011901 NIDCD NIH HHS , R01 DE018824 NIDCR NIH HHS, R01 HD054354 NICHD NIH HHS , MRC_MC_U117560482 Medical Research Council