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XB-ART-45000
PLoS One. January 1, 2012; 7 (3): e32635.

CRIM1 complexes with ß-catenin and cadherins, stabilizes cell-cell junctions and is critical for neural morphogenesis.

Ponferrada VG , Fan J , Vallance JE , Hu S , Mamedova A , Rankin SA , Kofron M , Zorn AM , Hegde RS , Lang RA .


Abstract
In multicellular organisms, morphogenesis is a highly coordinated process that requires dynamically regulated adhesion between cells. An excellent example of cellular morphogenesis is the formation of the neural tube from the flattened epithelium of the neural plate. Cysteine-rich motor neuron protein 1 (CRIM1) is a single-pass (type 1) transmembrane protein that is expressed in neural structures beginning at the neural plate stage. In the frog Xenopus laevis, loss of function studies using CRIM1 antisense morpholino oligonucleotides resulted in a failure of neural development. The CRIM1 knockdown phenotype was, in some cases, mild and resulted in perturbed neural fold morphogenesis. In severely affected embryos there was a dramatic failure of cell adhesion in the neural plate and complete absence of neural structures subsequently. Investigation of the mechanism of CRIM1 function revealed that it can form complexes with ß-catenin and cadherins, albeit indirectly, via the cytosolic domain. Consistent with this, CRIM1 knockdown resulted in diminished levels of cadherins and ß-catenin in junctional complexes in the neural plate. We conclude that CRIM1 is critical for cell-cell adhesion during neural development because it is required for the function of cadherin-dependent junctions.

PubMed ID: 22427856
PMC ID: PMC3299674
Article link: PLoS One.
Grant support: (R01 EY019377 NEI NIH HHS , R01 EY014648 NEI NIH HHS , R01 EY016241 NEI NIH HHS , R01 DK070858 NIDDK NIH HHS

Genes referenced: casp3 cdh1 cdh2 cdh3 crim1 ctnnb1 odc1 tubb2b
Antibodies referenced: Cdh1 Ab1 Cdh3 Ab1 Ctnnb1 Ab2 FLAG Ab1
Article Images: [+] show captions

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