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XB-ART-45093
Cell February 17, 2012; 148 (4): 752-64.

Local translation of extranuclear lamin B promotes axon maintenance.

Yoon BC , Jung H , Dwivedy A , O'Hare CM , Zivraj KH , Holt CE .


Abstract
Local protein synthesis plays a key role in regulating stimulus-induced responses in dendrites and axons. Recent genome-wide studies have revealed that thousands of different transcripts reside in these distal neuronal compartments, but identifying those with functionally significant roles presents a challenge. We performed an unbiased screen to look for stimulus-induced, protein synthesis-dependent changes in the proteome of Xenopus retinal ganglion cell (RGC) axons. The intermediate filament protein lamin B2 (LB2), normally associated with the nuclear membrane, was identified as an unexpected major target. Axonal ribosome immunoprecipitation confirmed translation of lb2 mRNA in vivo. Inhibition of lb2 mRNA translation in axons in vivo does not affect guidance but causes axonal degeneration. Axonal LB2 associates with mitochondria, and LB2-deficient axons exhibit mitochondrial dysfunction and defects in axonal transport. Our results thus suggest that axonally synthesized lamin B plays a crucial role in axon maintenance by promoting mitochondrial function.

PubMed ID: 22341447
PMC ID: PMC3314965
Article link: Cell
Grant support: [+]
Genes referenced: actl6a atoh7 bdnf casp3.2 cox4i1 en1 lmnb1 lmnb2 ndel1 nf1 pax6 pcdh7 pou4f2 tec ugcg vdac2 zic1
Morpholinos: lmnb2 MO1


Article Images: [+] show captions
References [+] :
Aakalu, Dynamic visualization of local protein synthesis in hippocampal neurons. 2001, Pubmed


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