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XB-ART-45353
Dev Dyn August 1, 2012; 241 (8): 1260-73.

Regulation of early xenopus embryogenesis by Smad ubiquitination regulatory factor 2.

Das S , Chang C .


Abstract
Smad ubiquitination regulatory factor (Smurf) 1 and 2 are E3 ubiquitin ligases originally identified as inhibitors of transforming growth factor beta signaling and are shown to modulate multiple cellular activities. The roles of Smurfs in vertebrate embryogenesis, however, are not completely understood. Here we investigate the function of Smurf2 during early Xenopus development. We show that distinctly from Smurf1, overexpression of Smurf2 in presumptive mesoderm interfered with mesoderm induction and caused axial defects, whereas knockdown of Smurf2 with antisense morpholino oligonucleotides resulted in expansion of the mesoderm. These results imply that Smurf2 may modulate nodal-mediated mesodermal induction. Consistently, ventral expression of Smurf2 induced a partial secondary axis with head structures. In the ectoderm, Smurf2 resembled Smurf1 in controlling neural and epidermal marker expression and influencing head formation. Smurf1, but not Smurf2, additionally affected neural tube closure. Interestingly, both Smurfs could enhance as well as repress neural crest markers, implying that they modulate their targets dynamically during neural plate border specification. Our data demonstrate that Smurf1 and Smurf2 have overlapping and distinct functionalities during early frog embryogenesis; collectively, they regulate ectodermal and mesodermal induction and patterning to ensure normal development of Xenopus embryos.

PubMed ID: 22674516
PMC ID: PMC3399951
Article link: Dev Dyn
Grant support: [+]
Genes referenced: chrd.1 krt70 nodal nodal1 nrp1 pax3 pax6 smurf1 smurf2 snai2 sox2 tbxt wnt8a
Antibodies: Smurf1 Ab2 Smurf2 Ab1
Morpholinos: smurf1 MO1 smurf2 MO2


Article Images: [+] show captions
References:
Alexandrova, 2006, Pubmed, Xenbase [+]


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