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XB-ART-45452
PLoS One January 1, 2012; 7 (6): e38939.

Xenopus as a model system for the study of GOLPH2/GP73 function: Xenopus GOLPH2 is required for pronephros development.

Li L , Wen L , Gong Y , Mei G , Liu J , Chen Y , Peng T .


Abstract
GOLPH2 is a highly conserved protein. It is upregulated in a number of tumors and is being considered as an emerging biomarker for related diseases. However, the function of GOLPH2 remains unknown. The Xenopus model is used to study the function of human proteins. We describe the isolation and characterization of Xenopus golph2, which dimerizes and localizes to the Golgi in a manner similar to human GOLPH2. Xenopus golph2 is expressed in the pronephros during early development. The morpholino-mediated knockdown of golph2 results in edema formation. Additionally, Nephrin expression is enhanced in the glomus, and the expression of pronephric marker genes, such as atp1b1, ClC-K, NKCC2, and NBC1, is diminished in the tubules and duct. Expression patterns of the transcription factors WT1, Pax2, Pax8, Lim1, GATA3, and HNF1β are also examined in the golph2 knockdown embryos, the expression of WT1 is increased in the glomus and expanded laterally in the pronephric region. We conclude that the deletion of golph2 causes an increase in the expression of WT1, which may promote glomus formation and inhibit pronephric tubule differentiation.

PubMed ID: 22719994
PMC ID: PMC3375297
Article link: PLoS One

Genes referenced: actb actl6a atp1b1 clcnkb gata3 golm1 hnf1a hnf1b lhx1 nphs1 odc1 pax2 pax8 slc12a1 slc4a4 slc5a1.2 wt1
GO keywords: Golgi apparatus part [+]
Morpholinos: golm1 MO1

Disease Ontology terms: prostate cancer [+]

Article Images: [+] show captions
References:
Bachert, 2007, Pubmed [+]


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