Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-45461
Dev Cell. April 17, 2012; 22 (4): 775-87.

Spindle position in symmetric cell divisions during epiboly is controlled by opposing and dynamic apicobasal forces.

Woolner S , Papalopulu N .


Abstract
Orientation of cell division is a vital aspect of tissue morphogenesis and growth. Asymmetric divisions generate cell fate diversity and epithelial stratification, whereas symmetric divisions contribute to tissue growth, spreading, and elongation. Here, we describe a mechanism for positioning the spindle in symmetric cell divisions of an embryonic epithelium. We show that during the early stages of epiboly, spindles in the epithelium display dynamic behavior within the plane of the epithelium but are kept firmly within this plane to give a symmetric division. This dynamic stability relies on balancing counteracting forces: an apically directed force exerted by F-actin/myosin-2 via active cortical flow and a basally directed force mediated by microtubules and myosin-10. When both forces are disrupted, spindle orientation deviates from the epithelial plane, and epithelial surface is reduced. We propose that this dynamic mechanism maintains symmetric divisions while allowing the quick adjustment of division plane to facilitate even tissue spreading.

PubMed ID: 22406140
PMC ID: PMC3332010
Article link: Dev Cell.
Grant support: Wellcome Trust

Genes referenced: ccrn4l ctrl hist2h2be myh4 myh6 myo10 nr2e1 rho tjp1
Antibodies referenced:
Article Images: [+] show captions

My Xenbase: [ Log-in / Register ]
version: [3.2.1]


Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556