Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Invest Ophthalmol Vis Sci
2012 Apr 24;534:2127-32. doi: 10.1167/iovs.11-8471.
Show Gene links
Show Anatomy links
Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro.
Baetz NW
,
Stamer WD
,
Yool AJ
.
???displayArticle.abstract???
The retinal pigment epithelium (RPE) expresses aquaporin-1 (AQP1) and components of the natriuretic peptide signaling pathway. We hypothesized that stimulation of the natriuretic signaling pathway in RPE with atrial natriuretic peptide (ANP) and with membrane-permeable analogs of cGMP would induce a net apical-to-basal transport of fluid. The hypothesis was tested using human RPE cultures that retain properties seen in vivo. Confluent monolayers were treated with ANP or membrane-permeable cGMP analogs in the presence of anantin, H-8, and an AQP1 inhibitor, AqB013. Fluid movement from the apical to basal chambers was measured by weight and used to calculate net fluid transport. Our results demonstrated a 40% increase in net apical-to-basal fluid transport by ANP (5 μM) that was inhibited completely by the ANP receptor antagonist anantin and a 60% increase in net apical-to-basal fluid transport in response to the extracellularly applied membrane-permeable cGMP analog pCPT-cGMP (50 μM), which was not affected by the protein kinase G inhibitor H-8. The aquaporin antagonist AqB013 (20 μM) inhibited the cGMP-stimulated RPE fluid flux. The effect of cGMP is consistent with an enhancement of the net fluid flux in RPE mediated by AQP1 channels. Pharmacologic activation of cGMP signaling and concomitant stimulation of fluid uptake from the subretinal space could offer insights into a new approach to treating or reducing the risk of retinal detachment.
Adorante,
Potassium-dependent volume regulation in retinal pigment epithelium is mediated by Na,K,Cl cotransport.
1990, Pubmed
Adorante,
Potassium-dependent volume regulation in retinal pigment epithelium is mediated by Na,K,Cl cotransport.
1990,
Pubmed
Amiry-Moghaddam,
An alpha-syntrophin-dependent pool of AQP4 in astroglial end-feet confers bidirectional water flow between blood and brain.
2003,
Pubmed
Anthony,
Cloned human aquaporin-1 is a cyclic GMP-gated ion channel.
2000,
Pubmed
,
Xenbase
Bialek,
K+ and Cl- transport mechanisms in bovine pigment epithelium that could modulate subretinal space volume and composition.
1994,
Pubmed
Boassa,
Single amino acids in the carboxyl terminal domain of aquaporin-1 contribute to cGMP-dependent ion channel activation.
2003,
Pubmed
,
Xenbase
Boassa,
Physiological roles of aquaporins in the choroid plexus.
2005,
Pubmed
Boassa,
Ion channel function of aquaporin-1 natively expressed in choroid plexus.
2006,
Pubmed
Bringmann,
Neuronal versus glial cell swelling in the ischaemic retina.
2005,
Pubmed
Clemo,
cGMP and atrial natriuretic factor regulate cell volume of rabbit atrial myocytes.
1995,
Pubmed
Denker,
Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules.
1988,
Pubmed
Diederen,
Cyclic GMP synthesis by human retinal pigment epithelial cells is mainly mediated via the particulate guanylyl cyclase pathway.
2007,
Pubmed
Diederen,
Cyclic GMP in the pig vitreous and retina after experimental retinal detachment.
2008,
Pubmed
Diederen,
Two autocrine pathways to regulate cyclic GMP synthesis in cultured human retinal pigment epithelial cells.
2008,
Pubmed
Fernandez-Durango,
Messenger RNAs encoding the natriuretic peptides and their receptors are expressed in the eye.
1995,
Pubmed
Frambach,
The rate and route of fluid resorption from the subretinal space of the rabbit.
1982,
Pubmed
Hidaka,
Pharmacology of protein kinase inhibitors.
1992,
Pubmed
Hochgesand,
Catecholaminergic regulation of Na-K-Cl cotransport in pigmented ciliary epithelium: differences between PE and NPE.
2001,
Pubmed
Hooper,
New treatments in age-related macular degeneration.
2003,
Pubmed
Hu,
A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers.
2001,
Pubmed
Hu,
Localization of NaK ATPase on cultured human retinal pigment epithelium.
1994,
Pubmed
Hu,
Chloride transport in cultured fetal human retinal pigment epithelium.
1996,
Pubmed
Hughes,
Inwardly rectifying K+ currents in isolated human retinal pigment epithelial cells.
1996,
Pubmed
Johanson,
Atrial natriuretic peptide: its putative role in modulating the choroid plexus-CSF system for intracranial pressure regulation.
2006,
Pubmed
Kang,
Management of retinal detachment: a guide for non-ophthalmologists.
2008,
Pubmed
King,
Pathophysiology of the aquaporin water channels.
1996,
Pubmed
La Heij,
Decreased levels of cGMP in vitreous and subretinal fluid from eyes with retinal detachment.
2003,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Li,
IFN{gamma} regulates retinal pigment epithelial fluid transport.
2009,
Pubmed
Maminishkis,
The P2Y(2) receptor agonist INS37217 stimulates RPE fluid transport in vitro and retinal reattachment in rat.
2002,
Pubmed
Marmor,
Pharmacologic modification of subretinal fluid absorption in the rabbit eye.
1986,
Pubmed
Marmor,
Conditions necessary for the formation of serous detachment. Experimental evidence from the cat.
1994,
Pubmed
Migliati,
Inhibition of aquaporin-1 and aquaporin-4 water permeability by a derivative of the loop diuretic bumetanide acting at an internal pore-occluding binding site.
2009,
Pubmed
,
Xenbase
Mikami,
Atrial natriuretic peptide stimulates Cl- transport in retinal pigment epithelial cells.
1995,
Pubmed
Miller,
Active ion transport pathways in the bovine retinal pigment epithelium.
1990,
Pubmed
Negi,
Effects of intraocular pressure and other factors on subretinal fluid resorption.
1987,
Pubmed
Nielsen,
Aquaporins in the kidney: from molecules to medicine.
2002,
Pubmed
Potter,
Natriuretic peptides, their receptors, and cyclic guanosine monophosphate-dependent signaling functions.
2006,
Pubmed
Rollín,
Natriuretic peptide system in the human retina.
2004,
Pubmed
Rowe,
Retinal detachment in Olmsted County, Minnesota, 1976 through 1995.
1999,
Pubmed
Schwede,
Cyclic nucleotide analogs as biochemical tools and prospective drugs.
2000,
Pubmed
Stamer,
Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement.
2003,
Pubmed
Stamer,
Localization of aquaporin CHIP in the human eye: implications in the pathogenesis of glaucoma and other disorders of ocular fluid balance.
1994,
Pubmed
Stamer,
Cultured human trabecular meshwork cells express aquaporin-1 water channels.
1995,
Pubmed
Stamer,
Characterization of the transmembrane orientation of aquaporin-1 using antibodies to recombinant fusion proteins.
1996,
Pubmed
Tremblay,
Biochemistry and physiology of the natriuretic peptide receptor guanylyl cyclases.
2002,
Pubmed
Weber,
Anantin--a peptide antagonist of the atrial natriuretic factor (ANF). I. Producing organism, fermentation, isolation and biological activity.
1991,
Pubmed
Wimmers,
Ion channels in the RPE.
2007,
Pubmed
Wolfensberger,
Natriuretic peptides and their receptors in human neural retina and retinal pigment epithelium.
1994,
Pubmed
Yao,
Retinal adhesiveness is weakened by enzymatic modification of the interphotoreceptor matrix in vivo.
1990,
Pubmed
Yool,
Functional domains of aquaporin-1: keys to physiology, and targets for drug discovery.
2007,
Pubmed
Yu,
Mechanism of gating and ion conductivity of a possible tetrameric pore in aquaporin-1.
2006,
Pubmed
