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XB-ART-45654
Cell Rep June 28, 2012; 1 (6): 730-40.

A developmental requirement for HIRA-dependent H3.3 deposition revealed at gastrulation in Xenopus.

Szenker E , Lacoste N , Almouzni G .


Abstract
Discovering how histone variants that mark distinct chromatin regions affect a developmental program is a major challenge in the epigenetics field. To assess the importance of the H3.3 histone variant and its dedicated histone chaperone HIRA, we used an established developmental model, Xenopus laevis. After the early rapid divisions exploiting a large maternal pool of both replicative H3.2 and replacement H3.3, H3.3 transcripts show a distinct peak of expression at gastrulation. Depletion of both H3.2 and H3.3 leads to an early gastrulation arrest. However, with only H3.3 depletion, defects occur at late gastrulation, impairing further development. Providing exogenous H3.3 mRNAs, but not replicative H3.2 mRNAs, rescues these defects. Notably, downregulation of the H3.3 histone chaperone HIRA similarly impairs late gastrulation, and we find a global defect in H3.3 incorporation into chromatin comparable to H3.3 depletion. We discuss how specific HIRA-dependent H3.3 deposition is required for chromatin dynamics during gastrulation.

PubMed ID: 22813747
Article link: Cell Rep

Genes referenced: a2m abl1 actl6a admp bmp4 cer1 chrd.1 eomes fgf4 fgf8 gdf3 gsc h3f3a h3f3b hira hist1h2ad hist1h3g mix1 mixer myod1 nodal2 nog otx2 sia1 sox17a sox2 tbxt ventx1.2 ventx2.2 wnt11b zeb2 zic3
Antibodies: h3f3b Ab1 hist1h3g Ab1
Morpholinos: chaf1a MO1 h3f3a MO3 hira MO1 hist1h3g MO1 wdr5 MO2


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