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XB-ART-45704
Dev Dyn September 1, 2012; 241 (9): 1487-505.

Microarray-based identification of Pitx3 targets during Xenopus embryogenesis.

Hooker L , Smoczer C , KhosrowShahian F , Wolanski M , Crawford MJ .


Abstract
Unexpected phenotypes resulting from morpholino-mediated translational knockdown of Pitx3 in Xenopus laevis required further investigation regarding the genetic networks in which the gene might play a role. Microarray analysis was, therefore, used to assess global transcriptional changes downstream of Pitx3.From the large data set generated, selected candidate genes were confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization.We have identified four genes as likely direct targets of Pitx3 action: Pax6, β Crystallin-b1 (Crybb1), Hes7.1, and Hes4. Four others show equivocal promise worthy of consideration: Vent2, and Ripply2 (aka Ledgerline or Stripy), eFGF and RXRα. We also describe the expression pattern of additional and novel genes that are Pitx3-sensitive but that are unlikely to be direct targets.

PubMed ID: 22826267
Article link: Dev Dyn

Genes referenced: arsl baz2b bix1.3 cryba4 crybb1 crybb3 fgf4 gsc hes4 hes7.1 hes7.2 hoxa10 hoxa11 hoxa13 lgals1.1 lgals9 lhx1 lhx5 nrl obscn pax6 pitx3 ptgds rax rbp4l rdh10 rdh16 ripply2.1 ripply2.2 rxra sp5 sp5l tbx2 tbx4 tbx5 ttn ventx2.2 wnt1
Morpholinos: pitx3 MO1


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