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J Biol Chem September 7, 2012; 287 (37): 31342-8.

The role of heterodimeric AP-1 protein comprised of JunD and c-Fos proteins in hematopoiesis.

Lee SY , Yoon J , Lee MH , Jung SK , Kim DJ , Bode AM , Kim J , Dong Z .

Activator protein-1 (AP-1) regulates a wide range of cellular processes including proliferation, differentiation, and apoptosis. As a transcription factor, AP-1 is commonly found as a heterodimer comprised of c-Jun and c-Fos proteins. However, other heterodimers may also be formed. The function of these dimers, specifically the heterodimeric AP-1 comprised of JunD and c-Fos (AP-1(JunD/c-Fos)), has not been elucidated. Here, we identified a function of AP-1(JunD/c-Fos) in Xenopus hematopoiesis. A gain-of-function study performed by overexpressing junD and c-fos and a loss-of-function study using morpholino junD demonstrate a critical role for AP-1(JunD/c-Fos) in hematopoiesis during Xenopus embryogenesis. Additionally, we confirmed that JunD of AP-1(JunD/c-Fos) is required for BMP-4-induced hematopoiesis. We also demonstrated that BMP-4 regulated JunD activity at the transcriptional regulation and post-translational modification levels. Collectively, our findings identify AP-1(JunD/c-Fos) as a novel hematopoietic transcription factor and the requirement of AP-1(JunD/c-Fos) in BMP-4-induced hematopoiesis during Xenopus hematopoiesis.

PubMed ID: 22822070
PMC ID: PMC3438963
Article link: J Biol Chem
Grant support: [+]
Genes referenced: actl6a bmp4 fos jun jund jund.2 klf17 klf4 lmo2 tal1
Antibodies: FLAG Ab1 HA Ab3 Jun Ab1
Morpholinos: jun MO1 jun MO2 jund MO1 jund MO2

Phenotypes: Xla Wt + fos + jund (fig.2.c) [+]

Article Images: [+] show captions
References [+] :
Ameyar, A role for AP-1 in apoptosis: the case for and against. 2003, Pubmed

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