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FEMS Yeast Res
2012 Jun 01;124:486-90. doi: 10.1111/j.1567-1364.2012.00794.x.
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Treslin, DUE-B, and GEMC1 cannot complement Sld3 mutants in fission yeast.
Wang Z
,
Kim E
,
Leffak M
,
Xu YJ
.
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Initiation of DNA replication in eukaryotes is an evolutionarily conserved process that involves two distinct steps: the formation of prereplication complexes at replication origins in G1 and the assembly of preinitiation complexes (pre-ICs) in S phase, which leads to activation of the replication helicase. For the assembly of pre-ICs in yeast, formation of the Sld2-Dpb11-Sld3 complex is a critical event that requires phosphorylation of Sld2 and Sld3 by cyclin-dependent kinase. In mammals, RecQL4 and TopBP1 are excellent ortholog candidates for Sld2 and Dpb11, respectively. In this past year, three TopBP1-interacting proteins Treslin/Ticrr, GEMC1, and DUE-B have been identified in metazoans as possible functional orthologs of the yeast Sld3. To test this hypothesis, we carried out several complementation tests in fission yeast. The proteins were expressed at various levels in the temperature-sensitive sld3-10 mutant and in cells that lack endogenous Sld3. Our result showed that none of these metazoan proteins could rescue growth defect of the sld3 mutants. Although the result may have several interpretations, it is possible that the helicase activation in mammals has diverged in complexity during evolution from that in yeasts and may involve multiple players that interact with TopBP1.
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22380713
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Balestrini,
GEMC1 is a TopBP1-interacting protein required for chromosomal DNA replication.
2010, Pubmed,
Xenbase
Balestrini,
GEMC1 is a TopBP1-interacting protein required for chromosomal DNA replication.
2010,
Pubmed
,
Xenbase
Boos,
Regulation of DNA replication through Sld3-Dpb11 interaction is conserved from yeast to humans.
2011,
Pubmed
Casper,
The c-myc DNA-unwinding element-binding protein modulates the assembly of DNA replication complexes in vitro.
2005,
Pubmed
,
Xenbase
Chowdhury,
The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation.
2010,
Pubmed
,
Xenbase
Heller,
Eukaryotic origin-dependent DNA replication in vitro reveals sequential action of DDK and S-CDK kinases.
2011,
Pubmed
Ilves,
Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.
2010,
Pubmed
Kamimura,
Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae.
2001,
Pubmed
Kumagai,
Treslin collaborates with TopBP1 in triggering the initiation of DNA replication.
2010,
Pubmed
,
Xenbase
Kumagai,
Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication.
2011,
Pubmed
,
Xenbase
Langan,
Mammalian growth-associated H1 histone kinase: a homolog of cdc2+/CDC28 protein kinases controlling mitotic entry in yeast and frog cells.
1989,
Pubmed
,
Xenbase
Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
,
Pubmed
Masai,
Eukaryotic chromosome DNA replication: where, when, and how?
2010,
Pubmed
Mueller,
DNA replication: mammalian Treslin-TopBP1 interaction mirrors yeast Sld3-Dpb11.
2011,
Pubmed
Nakajima,
SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.
2002,
Pubmed
Sanchez-Pulido,
Homology explains the functional similarities of Treslin/Ticrr and Sld3.
2010,
Pubmed
Sangrithi,
Initiation of DNA replication requires the RECQL4 protein mutated in Rothmund-Thomson syndrome.
2005,
Pubmed
,
Xenbase
Sansam,
A vertebrate gene, ticrr, is an essential checkpoint and replication regulator.
2010,
Pubmed
Tanaka,
CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast.
2007,
Pubmed
Tanaka,
The role of CDK in the initiation step of DNA replication in eukaryotes.
2007,
Pubmed
Yamane,
Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA.
1999,
Pubmed
Zegerman,
Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast.
2007,
Pubmed
