Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Phytochemistry
2011 Dec 01;7218:2385-95. doi: 10.1016/j.phytochem.2011.08.014.
Show Gene links
Show Anatomy links
Identification of GABA A receptor modulators in Kadsura longipedunculata and assignment of absolute configurations by quantum-chemical ECD calculations.
Zaugg J
,
Ebrahimi SN
,
Smiesko M
,
Baburin I
,
Hering S
,
Hamburger M
.
???displayArticle.abstract???
A petroleum ether extract of Kadsura longipedunculata enhanced the GABA-induced chloride current (I(GABA)) by 122.5±0.3% (n=2) when tested at 100 μg/ml in Xenopuslaevis oocytes expressing GABA A receptors (α(1)β(2)γ(2S) subtype) in two-microelectrode voltage clamp measurements. Thirteen compounds were subsequently identified by HPLC-based activity profiling as responsible for GABA A receptor activity and purified in preparative scale. 6-Cinnamoyl-6,7-dihydro-7-myrceneol and 5,6-dihydrocuparenic acid were thereby isolated for the first time. The determination of the absolute stereochemistry of these compounds was achieved by comparison of experimental and calculated ECD spectra. All but one of the 13 isolated compounds from K. longipedunculata potentiated I(GABA) through GABA A receptors composed of α(1)β(2)γ(2S) subunits in a concentration-dependent manner. Potencies ranged from 12.8±3.1 to 135.6±85.7 μM, and efficiencies ranged from 129.7±36.8% to 885.8±291.2%. The phytochemical profiles of petroleum ether extracts of Kadsura japonica fruits (114.1±2.6% potentiation of I(GABA) at 100 μg/ml, n=2), and Schisandra chinensis fruits (inactive at 100 μg/ml) were compared by HPLC-PDA-ESIMS with that of K. longipedunculata.
Adams,
A protocol for HPLC-based activity profiling for natural products with activities against tropical parasites.
2009, Pubmed
Adams,
A protocol for HPLC-based activity profiling for natural products with activities against tropical parasites.
2009,
Pubmed
Ai,
Honokiol and magnolol selectively interact with GABAA receptor subtypes in vitro.
2001,
Pubmed
Baburin,
Automated fast perfusion of Xenopus oocytes for drug screening.
2006,
Pubmed
,
Xenbase
Cheng,
Oxygenated lignans from the fruits of Schisandra arisanensis.
2009,
Pubmed
Danz,
Identification and isolation of the cyclooxygenase-2 inhibitory principle in Isatis tinctoria.
2001,
Pubmed
Dittmann,
HPLC-based activity profiling of Salvia miltiorrhiza for MAO A and iNOS inhibitory activities.
2004,
Pubmed
Huang,
Sedative and hypnotic activities of the ethanol fraction from Fructus Schisandrae in mice and rats.
2007,
Pubmed
Kamel,
Beyond polymaxenolide: Cembrane-africanane terpenoids from the hybrid soft coral Sinularia maxima x S. polydactyla.
2009,
Pubmed
Khom,
Pharmacological properties of GABAA receptors containing gamma1 subunits.
2006,
Pubmed
,
Xenbase
Kim,
HPLC-based activity profiling approach for the discovery of GABAA receptor ligands using an automated two microelectrode voltage clamp assay on Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Li,
HPLC-based activity profiling for GABA(A) receptor modulators: a new dihydroisocoumarin from Haloxylon scoparium.
2010,
Pubmed
,
Xenbase
Lu,
Analysis of Schisandra chinensis and Schisandra sphenanthera.
2009,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Möhler,
The rise of a new GABA pharmacology.
2011,
Pubmed
Olsen,
International Union of Pharmacology. LXX. Subtypes of gamma-aminobutyric acid(A) receptors: classification on the basis of subunit composition, pharmacology, and function. Update.
2008,
Pubmed
Opletal,
Dibenzo[a,c]cyclooctadiene lignans of the genus Schisandra: importance, isolation and determination.
2004,
Pubmed
Pajouhesh,
Medicinal chemical properties of successful central nervous system drugs.
2005,
Pubmed
Panossian,
Pharmacology of Schisandra chinensis Bail.: an overview of Russian research and uses in medicine.
2008,
Pubmed
Pei,
[A study of the central pharmacological action of piperine and its derivatives].
1980,
Pubmed
Potterat,
BI-32169, a bicyclic 19-peptide with strong glucagon receptor antagonist activity from Streptomyces sp.
2004,
Pubmed
Sadhu,
Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities.
2003,
Pubmed
Tayone,
Stereochemical investigations of isochromenones and isobenzofuranones isolated from Leptosphaeria sp. KTC 727.
2011,
Pubmed
Wang,
Chemical constituents of two Chinese Magnoliaceae plants, Tsoongiodendron odorum and Manglietiastrum sinicum, and their inhibition of platelet aggregation.
2000,
Pubmed
Wei,
A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera.
2010,
Pubmed
Xiao,
[Argument on the correct Chinese name of genus Kadsura Kaempf.ex Juss].
2010,
Pubmed
Yang,
HPLC-based activity profiling for GABAA receptor modulators from the traditional Chinese herbal drug Kushen (Sophora flavescens root).
2011,
Pubmed
,
Xenbase
Zaugg,
Identification and characterization of GABA(A) receptor modulatory diterpenes from Biota orientalis that decrease locomotor activity in mice.
2011,
Pubmed
,
Xenbase
Zaugg,
Positive GABA(A) receptor modulators from Acorus calamus and structural analysis of (+)-dioxosarcoguaiacol by 1D and 2D NMR and molecular modeling.
2011,
Pubmed
,
Xenbase
Zaugg,
HPLC-based activity profiling of Angelica pubescens roots for new positive GABAA receptor modulators in Xenopus oocytes.
2011,
Pubmed
,
Xenbase
Zaugg,
HPLC-based activity profiling: discovery of piperine as a positive GABA(A) receptor modulator targeting a benzodiazepine-independent binding site.
2010,
Pubmed
,
Xenbase
da Silva,
Aryltetralone lignans and 7,8-seco-lignans from Holostylis reniformis.
2004,
Pubmed
