Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The pipid frog Xenopus tropicalis has emerged as a powerful new model system for combining genetic and genomic analysis of tetrapod development with robust embryological, molecular, and biochemical assays. Its early development closely resembles that of its well-understood relative X. laevis, from which techniques and reagents can be readily transferred. In contrast to the tetraploid X. laevis, X. tropicalis has a compact diploid genome with strong synteny to those of amniotes. Recently, advances in high-throughput sequencing together with solution-hybridization whole-exome enrichment technology offer powerful strategies for cloning novel mutations as well as reverse genetic identification of sequence lesions in specific genes of interest. Further advantages include the wide range of functional and molecular assays available, the large number of embryos/meioses produced, and the ease of haploid genetics and gynogenesis. The addition of these genetic tools to X. tropicalis provides a uniquely flexible platform for analysis of gene function in vertebrate development.
Abu-Daya,
The secreted integrin ligand nephronectin is necessary for forelimb formation in Xenopus tropicalis.
2011, Pubmed,
Xenbase
Abu-Daya,
The secreted integrin ligand nephronectin is necessary for forelimb formation in Xenopus tropicalis.
2011,
Pubmed
,
Xenbase
Abu-Daya,
Absence of heartbeat in the Xenopus tropicalis mutation muzak is caused by a nonsense mutation in cardiac myosin myh6.
2009,
Pubmed
,
Xenbase
Allen,
Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.
2005,
Pubmed
,
Xenbase
Amaya,
Xenomics.
2005,
Pubmed
,
Xenbase
Bahary,
The Zon laboratory guide to positional cloning in zebrafish.
2004,
Pubmed
Bassam,
Fast and sensitive silver staining of DNA in polyacrylamide gels.
1991,
Pubmed
Bewick,
Evolution of the closely related, sex-related genes DM-W and DMRT1 in African clawed frogs (Xenopus).
2011,
Pubmed
,
Xenbase
Carruthers,
Genetic and genomic prospects for Xenopus tropicalis research.
2006,
Pubmed
,
Xenbase
Chae,
Inducible control of tissue-specific transgene expression in Xenopus tropicalis transgenic lines.
2002,
Pubmed
,
Xenbase
Chang,
The nonsense-mediated decay RNA surveillance pathway.
2007,
Pubmed
Doetschman,
Targetted correction of a mutant HPRT gene in mouse embryonic stem cells.
,
Pubmed
Doyon,
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases.
2008,
Pubmed
Driever,
A genetic screen for mutations affecting embryogenesis in zebrafish.
1996,
Pubmed
ELSDALE,
A description of the technique for nuclear transplantation in Xenopus laevis.
1960,
Pubmed
,
Xenbase
Geach,
Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker.
2010,
Pubmed
,
Xenbase
Goda,
Genetic screens for mutations affecting development of Xenopus tropicalis.
2006,
Pubmed
,
Xenbase
Grammer,
Identification of mutants in inbred Xenopus tropicalis.
2005,
Pubmed
,
Xenbase
Gurdon,
The introduction of Xenopus laevis into developmental biology: of empire, pregnancy testing and ribosomal genes.
2000,
Pubmed
,
Xenbase
Haffter,
The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio.
1996,
Pubmed
Harland,
Xenopus research: metamorphosed by genetics and genomics.
2011,
Pubmed
,
Xenbase
Hartley,
Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system.
2002,
Pubmed
,
Xenbase
Hellsten,
The genome of the Western clawed frog Xenopus tropicalis.
2010,
Pubmed
,
Xenbase
Hirsch,
Xenopus tropicalis transgenic lines and their use in the study of embryonic induction.
2002,
Pubmed
,
Xenbase
Khokha,
Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.
2009,
Pubmed
,
Xenbase
Kimmel,
Genetics and early development of zebrafish.
1989,
Pubmed
Lehman,
Homologous recombination catalyzed by a nuclear extract from Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Neff,
dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics.
1998,
Pubmed
Noramly,
A gynogenetic screen to isolate naturally occurring recessive mutations in Xenopus tropicalis.
2005,
Pubmed
,
Xenbase
Nüsslein-Volhard,
Mutations affecting segment number and polarity in Drosophila.
1980,
Pubmed
Ogino,
Highly efficient transgenesis in Xenopus tropicalis using I-SceI meganuclease.
2006,
Pubmed
,
Xenbase
Postlethwait,
Zebrafish comparative genomics and the origins of vertebrate chromosomes.
2000,
Pubmed
Reinschmidt,
Gene-centromere mapping in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Ryffel,
Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus.
2003,
Pubmed
,
Xenbase
Sargent,
Cryopreservation of sperm of Xenopus laevis and Xenopus tropicalis.
2005,
Pubmed
,
Xenbase
Showell,
A comparative survey of the frequency and distribution of polymorphism in the genome of Xenopus tropicalis.
2011,
Pubmed
,
Xenbase
Streisinger,
Production of clones of homozygous diploid zebra fish (Brachydanio rerio).
1981,
Pubmed
Streisinger,
Segregation analyses and gene-centromere distances in zebrafish.
1986,
Pubmed
Thomas,
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.
1987,
Pubmed
Tompkins,
Experimentally induced homozygosity in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Vos,
AFLP: a new technique for DNA fingerprinting.
1995,
Pubmed
Waldner,
Transgenic Xenopus laevis strain expressing cre recombinase in muscle cells.
2006,
Pubmed
,
Xenbase
Wang,
A homeotic gene cluster patterns the anteroposterior body axis of C. elegans.
1993,
Pubmed
Wells,
A genetic map of Xenopus tropicalis.
2011,
Pubmed
,
Xenbase
Yergeau,
Injection-mediated transposon transgenesis in Xenopus tropicalis and the identification of integration sites by modified extension primer tag selection (EPTS) linker-mediated PCR.
2007,
Pubmed
,
Xenbase
Yergeau,
Remobilization of Tol2 transposons in Xenopus tropicalis.
2010,
Pubmed
,
Xenbase
Young,
Efficient targeted gene disruption in the soma and germ line of the frog Xenopus tropicalis using engineered zinc-finger nucleases.
2011,
Pubmed
,
Xenbase
