Xenopus tropicalis as a model organism for genetics and genomics: past, present, and future.
Xenopus tropicalis was introduced as a model system for genetic, and then genomic research, in the early 1990s, complementing work on the widely used model organism Xenopus laevis. Its shorter generation time and diploid genome has facilitated a number of experimental approaches. It has permitted multigenerational experiments (e.g., preparation of transgenic lines and generation of mutant lines) that have added powerful approaches for research by the Xenopus community. As a diploid animal, its simpler genome was sequenced before X. laevis, and has provided a highly valuable resource indispensable for all Xenopus researchers. As more sophisticated transgenic technologies for manipulating gene expression are developed, and mutations, particularly null mutations, are identified in widely studied genes involved in critical cellular and developmental processes, researchers will increasingly turn to X. tropicalis for definitive analysis of complex genetic pathways. This chapter describes the historical and conceptual development of X. tropicalis as a genetic and genomic model system for higher vertebrate development.
PubMed ID: 22956079
PMC ID: PMC3918953
Article link: Methods Mol Biol
Grant support: EY017400 NEI NIH HHS , EY019000 NEI NIH HHS , HD065713 NICHD NIH HHS , RR013221 NCRR NIH HHS , RR025867 NCRR NIH HHS , P40 OD010997 NIH HHS , R01 EY017400 NEI NIH HHS , R01 EY018000 NEI NIH HHS , R01 RR013221 NCRR NIH HHS , R21 HD065713 NICHD NIH HHS , R43 EY019000 NEI NIH HHS , R44 EY019000 NEI NIH HHS , P40 RR025867 NCRR NIH HHS