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Plant Physiol
2012 Oct 01;1602:965-77. doi: 10.1104/pp.112.203455.
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The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress.
Perrone I
,
Gambino G
,
Chitarra W
,
Vitali M
,
Pagliarani C
,
Riccomagno N
,
Balestrini R
,
Kaldenhoff R
,
Uehlein N
,
Gribaudo I
,
Schubert A
,
Lovisolo C
.
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We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;4N is the most expressed PIP2 gene in root. In situ hybridization confirmed root localization in the cortical parenchyma and close to the endodermis. We then constitutively overexpressed VvPIP2;4N in grape 'Brachetto', and in the resulting transgenic plants we analyzed (1) the expression of endogenous and transgenic VvPIP2;4N and of four other aquaporins, (2) whole-plant, root, and leaf ecophysiological parameters, and (3) leaf abscisic acid content. Expression of transgenic VvPIP2;4N inhibited neither the expression of the endogenous gene nor that of other PIP aquaporins in both root and leaf. Under well-watered conditions, transgenic plants showed higher stomatal conductance, gas exchange, and shoot growth. The expression level of VvPIP2;4N (endogenous + transgene) was inversely correlated to root hydraulic resistance. The leaf component of total plant hydraulic resistance was low and unaffected by overexpression of VvPIP2;4N. Upon water stress, the overexpression of VvPIP2;4N induced a surge in leaf abscisic acid content and a decrease in stomatal conductance and leaf gas exchange. Our results show that aquaporin-mediated modifications of root hydraulics play a substantial role in the regulation of water flow in well-watered grapevine plants, while they have a minor role upon drought, probably because other signals, such as abscisic acid, take over the control of water flow.
Aharon,
Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress.
2003, Pubmed
Aharon,
Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress.
2003,
Pubmed
Baiges,
Eight cDNA encoding putative aquaporins in Vitis hybrid Richter-110 and their differential expression.
2001,
Pubmed
Balestrini,
Differential expression of chitin synthase III and IV mRNAs in ascomata of Tuber borchii Vittad.
2000,
Pubmed
Barone,
Mercury-induced conformational changes and identification of conserved surface loops in plasma membrane aquaporins from higher plants. Topology of PMIP31 from Beta vulgaris L.
1997,
Pubmed
Barrowclough,
Radial hydraulic conductivity along developing onion roots.
2000,
Pubmed
Bevan,
Binary Agrobacterium vectors for plant transformation.
1984,
Pubmed
Biela,
The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.
1999,
Pubmed
,
Xenbase
Buckley,
The role of bundle sheath extensions and life form in stomatal responses to leaf water status.
2011,
Pubmed
Chaumont,
Plasma membrane intrinsic proteins from maize cluster in two sequence subgroups with differential aquaporin activity.
2000,
Pubmed
,
Xenbase
Choat,
Vascular function in grape berries across development and its relevance to apparent hydraulic isolation.
2009,
Pubmed
Chrispeels,
Aquaporins: the molecular basis of facilitated water movement through living plant cells?
1994,
Pubmed
Cochard,
Putative role of aquaporins in variable hydraulic conductance of leaves in response to light.
2007,
Pubmed
Cui,
Expression of the Vicia faba VfPIP1 gene in Arabidopsis thaliana plants improves their drought resistance.
2008,
Pubmed
Cutanda-Perez,
Ectopic expression of VlmybA1 in grapevine activates a narrow set of genes involved in anthocyanin synthesis and transport.
2009,
Pubmed
D'Angeli,
Osmotin induces cold protection in olive trees by affecting programmed cell death and cytoskeleton organization.
2007,
Pubmed
Fetter,
Interactions between plasma membrane aquaporins modulate their water channel activity.
2004,
Pubmed
,
Xenbase
Fouquet,
Identification of grapevine aquaporins and expression analysis in developing berries.
2008,
Pubmed
Galmés,
Aquaporin expression in response to different water stress intensities and recovery in Richter-110 (Vitis sp.): relationship with ecophysiological status.
2007,
Pubmed
Gambino,
Molecular characterization of grapevine plants transformed with GFLV resistance genes: I.
2005,
Pubmed
Gambino,
Transgene silencing in grapevines transformed with GFLV resistance genes: analysis of variable expression of transgene, siRNAs production and cytosine methylation.
2010,
Pubmed
Gelvin,
Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool.
2003,
Pubmed
Hachez,
Modulating the expression of aquaporin genes in planta: A key to understand their physiological functions?
2006,
Pubmed
Heckwolf,
The Arabidopsis thaliana aquaporin AtPIP1;2 is a physiologically relevant CO₂ transport facilitator.
2011,
Pubmed
Heinen,
Role of aquaporins in leaf physiology.
2009,
Pubmed
Hukin,
Sensitivity of cell hydraulic conductivity to mercury is coincident with symplasmic isolation and expression of plasmalemma aquaporin genes in growing maize roots.
2002,
Pubmed
Iocco,
Genetic transformation of major wine grape cultivars of Vitis vinifera L.
2001,
Pubmed
Johanson,
The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants.
2001,
Pubmed
Johansson,
Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation.
1998,
Pubmed
,
Xenbase
Kaldenhoff,
Aquaporins in plants.
2006,
Pubmed
Kaldenhoff,
Significance of plasmalemma aquaporins for water-transport in Arabidopsis thaliana.
1998,
Pubmed
,
Xenbase
Kaldenhoff,
Aquaporins and plant water balance.
2008,
Pubmed
Katsuhara,
Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants.
2003,
Pubmed
Kumar,
Gene stability in transgenic aspen (Populus). II. Molecular characterization of variable expression of transgene in wild and hybrid aspen.
2001,
Pubmed
Li,
Characterization of OsPIP2;7, a water channel protein in rice.
2008,
Pubmed
,
Xenbase
Liakoura,
Should structure-function relations be considered separately for homobaric vs. heterobaric leaves?
2009,
Pubmed
Lovisolo,
An abscisic acid-related reduced transpiration promotes gradual embolism repair when grapevines are rehydrated after drought.
2008,
Pubmed
Maghuly,
Molecular characterization of grapevine plants transformed with GFLV resistance genes: II.
2006,
Pubmed
Marjanović,
Aquaporins in poplar: what a difference a symbiont makes!
2005,
Pubmed
,
Xenbase
Martre,
Plasma membrane aquaporins play a significant role during recovery from water deficit.
2002,
Pubmed
Matsumoto,
Role of the aquaporin PIP1 subfamily in the chilling tolerance of rice.
2009,
Pubmed
,
Xenbase
Maurel,
Plant aquaporins: membrane channels with multiple integrated functions.
2008,
Pubmed
Moshelion,
Plasma membrane aquaporins in the motor cells of Samanea saman: diurnal and circadian regulation.
2002,
Pubmed
,
Xenbase
Otto,
Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from Nicotiana tabacum.
2000,
Pubmed
Otto,
Aquaporin tetramer composition modifies the function of tobacco aquaporins.
2010,
Pubmed
Peng,
Rhododendron catawbiense plasma membrane intrinsic proteins are aquaporins, and their over-expression compromises constitutive freezing tolerance and cold acclimation ability of transgenic Arabidopsis plants.
2008,
Pubmed
Postaire,
A PIP1 aquaporin contributes to hydrostatic pressure-induced water transport in both the root and rosette of Arabidopsis.
2010,
Pubmed
Sade,
Improving plant stress tolerance and yield production: is the tonoplast aquaporin SlTIP2;2 a key to isohydric to anisohydric conversion?
2009,
Pubmed
Sakurai,
Identification of 33 rice aquaporin genes and analysis of their expression and function.
2005,
Pubmed
Secchi,
Patterns of PIP gene expression in Populus trichocarpa during recovery from xylem embolism suggest a major role for the PIP1 aquaporin subfamily as moderators of refilling process.
2010,
Pubmed
,
Xenbase
Secchi,
Isolation and functional characterization of three aquaporins from olive (Olea europaea L.).
2007,
Pubmed
,
Xenbase
Siefritz,
PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants.
2002,
Pubmed
,
Xenbase
Sorieul,
Mechanisms and effects of retention of over-expressed aquaporin AtPIP2;1 in the endoplasmic reticulum.
2011,
Pubmed
Stoll,
Hormonal changes induced by partial rootzone drying of irrigated grapevine.
2000,
Pubmed
Tesniere,
Effects of genetic manipulation of alcohol dehydrogenase levels on the response to stress and the synthesis of secondary metabolites in grapevine leaves.
2006,
Pubmed
Thomas,
Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification.
1993,
Pubmed
Tournaire-Roux,
Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins.
2003,
Pubmed
,
Xenbase
Tsuchihira,
Effect of overexpression of radish plasma membrane aquaporins on water-use efficiency, photosynthesis and growth of Eucalyptus trees.
2010,
Pubmed
Tyree,
The dependence of leaf hydraulic conductance on irradiance during HPFM measurements: any role for stomatal response?
2005,
Pubmed
Vandeleur,
The role of plasma membrane intrinsic protein aquaporins in water transport through roots: diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine.
2009,
Pubmed
,
Xenbase
Yang,
Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool.
2008,
Pubmed
Zelazny,
An N-terminal diacidic motif is required for the trafficking of maize aquaporins ZmPIP2;4 and ZmPIP2;5 to the plasma membrane.
2009,
Pubmed
Zhang,
Indian mustard aquaporin improves drought and heavy-metal resistance in tobacco.
2008,
Pubmed
