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Mol Cell Biol. January 1, 2013; 33 (1): 48-58.

An unusual two-step control of CPEB destruction by Pin1.

Nechama M , Lin CL , Richter JD .

Cytoplasmic polyadenylation is a conserved mechanism that controls mRNA translation and stability. A key protein that promotes polyadenylation-induced translation of mRNAs in maturing Xenopus oocytes is the cytoplasmic polyadenylation element binding protein (CPEB). During this meiotic transition, CPEB is subjected to phosphorylation-dependent ubiquitination and partial destruction, which is necessary for successive waves of polyadenylation of distinct mRNAs. Here we identify the peptidyl-prolyl cis-trans isomerase Pin1 as an important factor mediating CPEB destruction. Pin1 interacts with CPEB in an unusual manner in which it occurs prior to CPEB phosphorylation and prior to Pin1 activation by serine 71 dephosphorylation. Upon induction of maturation, CPEB becomes phosphorylated, which occurs simultaneously with Pin1 dephosphorylation. At this time, the CPEB-Pin1 interaction requires cdk1-catalyzed CPEB phosphorylation on S/T-P motifs. Subsequent CPEB ubiquitination and destruction are mediated by a conformational change induced by Pin1 isomerization of CPEB. Similar to M phase progression in maturing Xenopus oocytes, the destruction of CPEB during the mammalian cell cycle requires Pin1 as well. These data identify Pin1 as a new and essential factor regulating CPEB degradation.

PubMed ID: 23090969
PMC ID: PMC3536308
Article link: Mol Cell Biol.
Grant support: GM46779 NIGMS NIH HHS , P30 DK32520 NIDDK NIH HHS , R01 GM046779 NIGMS NIH HHS , P30 DK032520 NIDDK NIH HHS

Genes referenced: cdk1 cpeb1 pin1

External Resources:

Abrahamsen, 2012, Pubmed[+]

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